文献类型：期刊文献

英文题名：Transcriptome and Degradome Profiling Reveals a Role of miR530 in the Circadian Regulation of Gene Expression in Kalanchoe marnieriana

作者：Hu, Zhikang[1,2,3] Nie, Ziyan[1,3] Yan, Chao[4] Huang, Hu[1,2] Ma, Xianjin[1,2] Wang, Yupeng[2] Ye, Ning[2] Tuskan, Gerald A.[5,6] Yang, Xiaohan[5,6] Yin, Hengfu[1,3]

第一作者：Hu, Zhikang

通信作者：Yin, HF[1];Yin, HF[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[2]Nanjing Forestry Univ, Coll Informat Sci & Technol, Nanjing 210037, Peoples R China;[3]Chinese Acad Forestry, Key Lab Forest Genet & Breeding, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[4]Chinese Acad Forestry, Expt Ctr Subtrop Forestry, Fenyi 336600, Peoples R China;[5]Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37830 USA;[6]Oak Ridge Natl Lab, DOE Ctr Bioenergy Innovat CBI, Oak Ridge, TN 37830 USA

年份：2021

卷号：10

期号：6

外文期刊名：CELLS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000665264500001)】；

基金：This work was supported by National Key R&D Program of China (2019YFD1000400) and National Science Foundation of China (31870578).

语种：英文

外文关键词：crassulacean acid metabolism; microRNA; photosynthesis; drought; circadian clock; Kalanchoe

摘要：Crassulacean acid metabolism (CAM) is an important photosynthetic pathway for plant adaptation to dry environments. CAM plants feature a coordinated interaction between mesophyll and epidermis functions that involves refined regulations of gene expression. Plant microRNAs (miRNAs) are crucial post-transcription regulators of gene expression, however, their roles underlying the CAM pathway remain poorly investigated. Here, we present a study characterizing the expression of miRNAs in an obligate CAM species Kalanchoe marnieriana. Through sequencing of transcriptome and degradome in mesophyll and epidermal tissues under the drought treatments, we identified differentially expressed miRNAs that were potentially involved in the regulation of CAM. In total, we obtained 84 miRNA genes, and eight of them were determined to be Kalanchoe-specific miRNAs. It is widely accepted that CAM pathway is regulated by circadian clock. We showed that miR530 was substantially downregulated in epidermal peels under drought conditions; miR530 targeted two tandem zinc knuckle/PLU3 domain encoding genes (TZPs) that were potentially involved in light signaling and circadian clock pathways. Our work suggests that the miR530-TZPs module might play a role of regulating CAM-related gene expression in Kalanchoe.