文献类型：期刊文献

英文题名：Full-length transcriptome sequencing analysis and development of EST-SSR markers for the endangered species Populus wulianensis

作者：Wu, Qichao[1] Zang, Fengqi[2] Xie, Xiaoman[3] Ma, Yan[1] Zheng, Yongqi[2] Zang, Dekui[1]

第一作者：Wu, Qichao

通信作者：Ma, Y[1]

机构：[1]Shandong Agr Univ, Coll Forestry, Key Lab State Forestry Adm Silviculture Lower Yel, Tai An 271018, Shandong, Peoples R China;[2]Chinese Acad Forestry, Inst Forestry, Beijing 100091, Peoples R China;[3]Ctr Forest Genet Resources Shandong Prov, Jinan 250014, Peoples R China

年份：2020

卷号：10

期号：1

外文期刊名：SCIENTIFIC REPORTS

收录：;Scopus(收录号：2-s2.0-85091777083);WOS:【SCI-EXPANDED(收录号:WOS:000577143400012)】；

基金：This study was supported by the Shandong Agricultural Seeds Engineering Project (2019LZGC01802) and the National Forest Germplasm Resources Platform Project 2019-2020. We would like to thank AJE (https://www.aje.cn/) for providing linguistic assistance during the preparation of this manuscript.

语种：英文

摘要：Populus wulianensis is an endangered species endemic to Shandong Province, China. Despite the economic and ornamental value of this species, few genomics and genetic studies have been performed. In this study, we performed a relevant analysis of the full-length transcriptome sequencing data of P. wulianensis and obtained expressed sequence tag (EST)-simple sequence repeat (SSR) markers with polymorphisms that can be used for further genetic research. In total, 8.18 Gb (3,521,665) clean reads with an average GC content of 42.12% were obtained. From the corrected 64,737 high-quality isoforms, 42,323 transcript sequences were obtained after redundancy analysis with CD-HIT. Among these transcript sequences, 41,876 sequences were annotated successfully. A total of 23,539 potential EST-SSRs were identified from 16,057 sequences. Excluding mononucleotides, the most abundant motifs were trinucleotide SSRs (47.80%), followed by di- (46.80%), tetra- (2.98%), hexa- (1.58%) and pentanucleotide SSRs (0.84%). Among the 100 designed EST-SSRs, 18 were polymorphic with high PIC values (0.721 and 0.683) and could be used for analyses of the genetic diversity and population structure of P. wulianensis. These full-length transcriptome sequencing data will facilitate gene discovery and functional genomics research in P. wulianensis, and the novel EST-SSRs developed in our study will promote molecular-assisted breeding, genetic diversity and conservation biology research in this species.