文献类型：期刊文献

英文题名：Development of Genomic SSR for the Subtropical Hardwood Tree Dalbergia hupeana and Assessment of Their Transferability to Other Related Species

作者：Li, Changhong[1] Zheng, Yongqi[1] Liu, Yu[1] Lin, Furong[1] Huang, Ping[1]

第一作者：Li, Changhong

通信作者：Huang, P[1]

机构：[1]Chinese Acad Forestry, Key Lab Forest Silviculture & Tree Cultivat, State Key Lab Tree Genet & Breeding, Natl Forestry & Grassland Adm,Res Inst Forestry, Beijing 100091, Peoples R China

年份：2021

卷号：12

期号：6

外文期刊名：FORESTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000665917400001)】；

基金：This research was funded by the National Natural Science Foundation of China (No.31761143002; No.3207141079) and Special Funds for the Laboratory of Forest Silviculture and Tree Cultivation, National Forestry and Grassland Administration (No. ZDRIF201713).

语种：英文

外文关键词：Dalbergia hupeana; genome survey; genomic SSRs; cross-species transferability; genetic diversity

摘要：Dalbergia hupeana Hance (D. hupeana) is a precious hardwood tree of the genus Dalbergia. It is one of the few species widely distributed within subtropical areas and is important for timber production and forest restoration. At present, there is little published genetic information on D. hupeana. Therefore, we performed a genome survey using next generation sequencing (NGS) and developed a set of novel genomic SSR (gSSR) markers from the assembled data, and assessed the transferability of these markers to other Dalbergia species in Asia. The results of the genome survey show the genome size of D. hupeana to be about 664 Mb and highly heterozygous. The assembly of sequencing data produced 2,431,997 contigs, and the initial assembly of the NGS data alone resulted in contig N50 of 393 kb with a total of 720 Mb. A total of 127,742 perfect SSR markers were found in the assembled contigs. A total of 37 highly polymorphic and easily genotyped gSSR markers were developed in D. hupeana, while the majority of gSSR markers could be successfully transferred to nine other Dalbergia species in Asia. The transferability rate of gSSR markers was highest in D. balansae, which is more closely related to D. hupeana. Seven gSSR markers were able to be amplified in all tested species. In addition, a preliminary assessment of the genetic diversity of three tree species in the Dalbergia genus suggested a high level of genetic diversity within populations distributed in the subtropical area in China. However, the determination of the global status of their genetic variation still requires further and more comprehensive assessment. Our findings will enable further studies on the genetic diversity, phylogenetics, germplasm characterization, and taxonomy of various Dalbergia species.