文献类型：期刊文献

中文题名：多花黄精嫩茎与根茎芽离体培养技术

英文题名：Tender stems and rhizome buds in vitro culture techniques in Polygonatum cyrtonema

作者：周新华[1] 曾满生[1] 肖智勇[2] 李峰卿[1] 周志兰[1] 钟秋平[1]

第一作者：周新华

机构：[1]中国林业科学研究院亚热带林业实验中心;[2]宜春市林业科学研究所

年份：2014

卷号：32

期号：4

起止页码：68-72

中文期刊名：经济林研究

外文期刊名：Nonwood Forest Research

收录：CSTPCD;;北大核心:【北大核心2011】；

基金：中央级公益性科研院所基本科研业务费专项资金(CAFYBB2012002)

语种：中文

中文关键词：多花黄精;芽增殖;组织培养

外文关键词：Polygonatum cyrtonema; bud propagation; tissue culture

分类号：S604.3

摘要：为给黄精规模化栽培提供可行的关键技术,利用江西分宜大岗山山区选育出的优良野生多花黄精嫩茎和根茎芽为试验材料,开展芽增殖离体组织培养试验,研究多花黄精的最佳灭菌方法、最佳外植体取材部位、最适基本培养基和最佳激素及质量浓度。试验结果表明:先用75%的酒精表面灭菌30 s,再用0.1%的氯化汞深层灭菌10 min,灭菌效果较好,外植体材料无菌保存率可达73.2%;最适基本培养基为MS培养基;最佳外植体选材部位为带芽根茎;最佳不定芽诱导培养基为MS+NAA1.5 mg/L+KT1.5 mg/L,平均每个根茎芽能诱导6.6个不定芽。
In order to provide get the feasible key techniques for large-scale cultivation, taking tender stems and rhizome buds in fine wild Polygonatum cyrtonema as research objects, which gathering from Dagangshan Mountains of Fenyi of Jiangxi Province, the bud propagation in vitro culture were carried out. The optimal sterilization method, the optimal explants in materials, the optimum basal culture medium, and the best combination and mass concentrations of hormones were researched. The results showed that the treatment of 75% alcohol for 30 s and 0.1% mercuric chloride for 10 min had better effect of sterilization, and the preservation rate of aseptic materials was up to 73.2%. MS was the optimal medium. Rhizome bud was the optimal explants part in P. cyrtonema. The optimal medium for inducing adventitious buds was MS medium with NAA1.5 mg/L and KT1.5 mg/L, and average number of buds induced from a rhizome bud is 6.6.