文献类型：期刊文献

中文题名：香石竹ACC氧化酶重复基因植物表达载体的构建及转基因植株的获得

英文题名：Construction of Repeated Carnation ACC Oxidase Gene PlantRecombinant Expression Vectors

作者：余义勋[1] 包满珠[1] 孙振元[2]

第一作者：余义勋

机构：[1]华中农业大学园艺林学学院;[2]中国林业科学研究院花卉中心

年份：2004

卷号：17

期号：1

起止页码：1-5

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金(39970532)项目;"948"项目(96 4 06)资助

语种：中文

中文关键词：香石竹;ACC氧化酶;植物表达载体;转基因植株;重复基因;切花

外文关键词：carnation( Dianthus caryophyllus L.);ACC oxidase(ACO);repeat gene;gene cloning;construction of recombinant vector;transformation

分类号：S681.5

摘要：香石竹ACC氧化酶基因从核NDA中克隆之后,将其首先构建成正义及反义的单拷贝植物表达载体,在此基础上又同向插入一个ACO基因片段,从而获得ACO的正义重复基因和反义重复基因植物表达载体,所得载体已通过酶切分析和PCR鉴定。将该表达载体导入根癌农杆菌LBA4404菌株,并转化香石竹品种Master、爱卡迪幼叶,经PCR检测和Southern杂交鉴定,获得了8株转化植珠。
The genomic fragment of carnation ACC oxidase,the key enzyme in ethylene biosynthesis,was cloned and single copy plant recombinant expression vectors were constructed first.The sense and antisense repeated plant recombinant expression vectors were constructed based on the single copy vectors.The repeated vectors were indentified by restriction enzyme and PCR analysis.The vectors were transformed into Agrobacterium tumefaciens ,which was confirmed by PCR analysis.Transgenic plants of carnation were obtained by using those vectors,which were confirmed by PCR analysis and Southern blotting.The transgenic plants were under further analysis for transgene silence effect.