文献类型：期刊文献

英文题名：Closely-related taxa influence woody species discrimination via DNA barcoding: evidence from global forest dynamics plots

作者：Pei, Nancai[1,2] Erickson, David L.[3] Chen, Bufeng[1,2] Ge, Xuejun[4] Mi, Xiangcheng[5] Swenson, Nathan G.[6] Zhang, Jin-Long[7] Jones, Frank A.[8] Huang, Chun-Lin[9] Ye, Wanhui[4] Hao, Zhanqing[10] Hsieh, Chang-Fu[11] Lum, Shawn[12] Bourg, Norman A.[13] Parker, John D.[14] Zimmerman, Jess K.[15] McShea, William J.[13] Lopez, Ida C.[3] Sun, I-Fang[16] Davies, Stuart J.[17] Ma, Keping[5] Kress, W. John[3]

第一作者：裴男才

通信作者：Pei, NC[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China;[3]Smithsonian Inst, Natl Museum Nat Hist, Dept Bot, Washington, DC 20013 USA;[4]Chinese Acad Sci, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China;[5]Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China;[6]Univ Maryland, Dept Biol, College Pk, MD 20742 USA;[7]Kadoorie Farm & Bot Garden, Flora Conservat Dept, Tai Po, Hong Kong, Peoples R China;[8]Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA;[9]Natl Museum Nat Sci, Dept Biol, Lab Mol Phylogenet, Taichung, Taiwan;[10]Chinese Acad Sci, Inst Appl Ecol, State Key Lab Forest & Soil Ecol, Shenyang 110016, Peoples R China;[11]Natl Taiwan Univ, Inst Ecol & Evolutionary Biol, Taipei 10764, Taiwan;[12]Nanyang Technol Univ, Natl Inst Educ, Singapore 637616, Singapore;[13]Smithsonian Conservat Biol Inst, Front Royal, VA USA;[14]Smithsonian Environm Res Ctr, Edgewater, MD 21037 USA;[15]Univ Puerto Rico, Inst Trop Ecosyst Studies, San Juan, PR 00936 USA;[16]Natl Dong Hwa Univ, Dept Nat Resources & Environm Studies, Hualien 97401, Taiwan;[17]Smithsonian Trop Res Inst, Forest Global Earth Observ, Ctr Trop Forest Sci, Washington, DC 20013 USA

年份：2015

卷号：5

外文期刊名：SCIENTIFIC REPORTS

收录：;Scopus(收录号：2-s2.0-84943799223);WOS:【SCI-EXPANDED(收录号:WOS:000362563500001)】；

基金：Funding was provided by the Special Fund for Forest Scientific Research in the Public Welfare (20140430105), NSF-China (31200471), the Ministry of Finance of the People's Republic of China (RITFYWZX201208), China Scholarship Council (201303270006), and CFERN & GENE Award Funds on Ecological Paper. We appreciate J.L., S.G., H.Y., N.Z., Y.F., Y.Z. and X.Y. for useful discussion, and thank many assistants of CTFS-ForestGEO FDPs in the field and technicians in the lab. We also thank the anonymous reviewers for their valuable comments on the manuscript.

语种：英文

摘要：To determine how well DNA barcodes from the chloroplast region perform in forest dynamics plots (FDPs) from global CTFS-ForestGEO network, we analyzed DNA barcoding sequences of 1277 plant species from a wide phylogenetic range (3 FDPs in tropics, 5 in subtropics and 5 in temperate zone) and compared the rates of species discrimination (RSD). We quantified RSD by two DNA barcode combinations (rbcL + matK and rbcL + matK + trnH-psbA) using a monophyly-based method (GARLI). We defined two indexes of closely-related taxa (G(m)/G(t) and S/G ratios) and correlated these ratios with RSD. The combination of rbcL + matK averagely discriminated 88.65%, 83.84% and 72.51% at the local, regional and global scales, respectively. An additional locus trnH-psbA increased RSD by 2.87%, 1.49% and 3.58% correspondingly. RSD varied along a latitudinal gradient and were negatively correlated with ratios of closely-related taxa. Successes of species discrimination generally depend on scales in global FDPs. We suggested that the combination of rbcL + matK + trnH-psbA is currently applicable for DNA barcoding-based phylogenetic studies on forest communities.