文献类型：期刊文献

英文题名：Changes of lignified-callus and wound-induced adventitious rooting in ancient Platycladus orientalis cuttings as affected by tree age

作者：Chang, Ermei[1,2] Guo, Wei[3] Xie, Yunhui[1] Jiang, Zeping[4] Dong, Yao[4] Jia, Zirui[1] Zhao, Xiulian[1] Liu, Jianfeng[1] Zhang, Jin[2]

第一作者：Chang, Ermei

通信作者：Liu, JF[1];Zhang, J[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, State Forestry Adm,Res Inst Forestry, Beijing 10091, Peoples R China;[2]Zhejiang A&F Univ, Coll Forestry & Biotechnol, State Key Lab Subtrop Silviculture, Hangzhou 311300, Zhejiang, Peoples R China;[3]Taishan Acad Forestry Sci, Tai An 271000, Shandong, Peoples R China;[4]Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Key Lab Forest Ecol Natl Forestry & Grassland Adm, Beijing 100091, Peoples R China

年份：2023

卷号：203

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001053176200001)】；

基金：This work was supported by the National Key Research and Development Program of China (2022YFD2200100-3) , the State Natural Science Fund Projects (31870664) , the State Key Laboratory of Sub-tropical Silviculture (SKLSS-KF2022-09) , and the National Non-Profit Research Institutions of the Chinese Academy of Forestry (CAFYBB2020ZD002-4) .

语种：英文

外文关键词：Platycladus orientalis; Secondary metabolism; Lignification; Adventitious root formation; Wounding

摘要：Stem cuttings of the endangered ancient Platycladus orientalis are a valuable genetic resource for cryopreservation and propagation, but their extremely hard adventitious root (AR) formation poses a challenge for regeneration. In this study, we investigated the causes of decreased AR formation during the initial stem (S1), callus expansion (S2), and AR formation (S3) stages in cuttings from 5-, 100-, and 700-year-old P. orientalis trees, and identified potential solutions to enhance AR formation. Proteomic analysis revealed an up-regulation of flavonoid and phenylpropanoid biosynthesis pathway-associated proteins, including chalcone synthase (CHS), chalcone isomerase (CHI), and flavonone-3-hydroxylase (F3H), as well as down-regulation of auxin transport-associated proteins, such as auxin transport protein (BIG) and auxin responsive (AIR12), in the S3 of 700-year-old donors cuttings. Subsequent biochemical analysis confirmed over-accumulation of flavonoids, phenolics, and lignin resulting in callus-lignification and inhibition of AR formation in 100-and 700-year-old donors. Notably, wounding of the lignified-callus significantly increased AR formation in cuttings of 100-year-old donors. The upregulation of expression of meristematic cells regulatory proteins (enolase (ENO) and elongation factor 1 & alpha; (EF1 & alpha;)), carbohydrate metabolism proteins (isoamylases (ISA)), and vitamin B6 biosynthesis proteins (pyridoxine synthase (PDX1) and pyridoxal kinase (PDXK)) after wounding, might promote AR formation in the lignified-callus with wounding of cuttings of 100-year-old donors. Overall, our findings reveal that calluslignification inhibits AR formation, while wounding promotes it in ancient P. orientalis. This study provides a foundation for enhancing the rooting rate and shortening the rooting time in ancient P. orientalis, and potentially other difficult-to-root species.