文献类型：期刊文献

英文题名：PcWRKY1 Represses Transcription of Yellow Stripe-Like 3 (PcYSL3) to Negatively Regulate Radial Cadmium Transport in Poplar Stems

作者：Chen, Xin[1] Zhang, Yuhong[1] Cheng, Yao[1] Yu, Wenjian[1] Yang, Lingyu[1] Shu, Peiqi[1] Zhou, Jing[1] Fayyaz, Payam[2] Luo, Zhi-Bin[1,3,4] Deng, Shurong[1] Shi, Wenguang[1]

第一作者：Chen, Xin

通信作者：Luo, ZB[1];Deng, SR[1];Shi, WG[1];Luo, ZB[2];Luo, ZB[3]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Univ Yasuj, Agr & Nat Resources Fac, Forest Range & Watershed Management Dept, Yasuj 7591963179, Iran;[3]Chinese Acad Forestry, Inst Ecol Conservat & Restorat, Beijing 100091, Peoples R China;[4]Chinese Acad Forestry Yellow River Delta, Comprehens Expt Ctr, Dongying 257000, Shandong, Peoples R China

年份：2025

卷号：12

期号：1

外文期刊名：ADVANCED SCIENCE

收录：;EI(收录号：20244617356686);Scopus(收录号：2-s2.0-85208545781);WOS:【SCI-EXPANDED(收录号:WOS:001354120500001)】；

基金：The platform at the facilities of the State Key Laboratory of Tree Genetics and Breeding is acknowledged. Funding: This work was jointly supported by grants from the National Natural Science Foundation of China (Grant nos. 32371841, 32371826, and 32371916) and the Key Research and Development Program of Shandong Province (Grant no. 2023CXPT026).

语种：英文

外文关键词：heavy metal; populus; transcriptional regulation; transporter; xylem-to-phloem transport

摘要：A considerable amount of cadmium (Cd) can accumulate in the bark of poplar stems, but the Cd transport pathway and its underlying molecular mechanisms remain unknown. Here, a Cd radial transport pathway in poplar stems and a previously unrecognized PcWRKY1-Yellow Stripe-Like 3 (PcYSL3) module that regulates Cd transport are identified in Populus x canescens (Aiton) Sm. Cadmiun-nicotianamine (Cd-NA) in xylem vessels in poplar stem-wood is unloaded to adjacent ray parenchyma cells and further radially transported to bark-phloem. PcYSL3 is putatively identified as involved in Cd radial transport in poplar stems. PcYSL3 is highly expressed in ray parenchyma cells adjacent to xylem vessels and the encoded protein localizes on the plasma membrane. Cd accumulation is greater in the wood and bark of PcYSL3-overexpressing poplars than the wild type, whereas the opposite is observed in PcYSL3-knockdown plants. PcWRKY1 can bind to the PcYSL3 promoter sequence and represses its expression. PcWRKY1 inhibits Cd accumulation in the wood and bark of plants. Thus, PcWRKY1 suppresses PcYSL3 transcription to negatively regulate Cd-NA unloading from xylem vessels to adjacent ray parenchyma cells and its radial transport in poplar stem. The findings have provided new insights into breeding of poplars for more effective remediation of heavy metal-contaminated soils.