文献类型：期刊文献

英文题名：Comparative Transcriptome Analysis of Flower Senescence of Camellia lutchuensis

作者：Liu, Weixin[1,2] Yin, Hengfu[1,2] Feng, Yi[1,2] Yu, Suhang[1,2] Fan, Zhengqi[1,2] Li, Xinlei[1,2] Li, Jiyuan[1,2]

第一作者：刘伟鑫

通信作者：Li, JY[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Tree Genet & Breeding, Hangzhou 311400, Zhejiang, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Forest Genet & Breeding, Hangzhou 311400, Zhejiang, Peoples R China

年份：2022

卷号：23

期号：1

起止页码：66-76

外文期刊名：CURRENT GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000811690000007)】；

基金：This work was funded by National Key R&D Program of China (Grant No. 2019YFD1001000).

语种：英文

外文关键词：Camellia lutchuensis; flower senescence; transcriptome; differentially expressed genes; ethylene; reactive oxygen species

摘要：Background: Flower senescence is the last stage of flower development and affects the ornamental and economic value of flower plants. There is still less known on flower senescence of the ornamental plant Camellia lutchuensis, a precious species of Camellia with significant commercial application value. Methods: Transcriptome sequencing was used to investigate the flower senescence in five developmental stages of C. lutchuensis. Results: By Illumina HiSeq sequencing, we generated approximately 101.16 Gb clean data and 46649 differentially expressed unigenes. Based on the different expression pattern, differentially expressed unigenes were classified into 10 Sub Class. And Sub Class 9 including 8252 unigenes, was highly expressed in the flower senescent stage, suggesting it had a potential regulatory relationship of flower senescence. First, we found that ethylene biosynthesis genes ACSs, ACOs, receptor ETR genes and signaling genes EINs, ERFs all upregulated during flower senescence, suggesting ethylene might play a key role in the flower senescence of C. lutchuensis. Furthermore, reactive oxygen species (ROS) production related genes peroxidase (POD), lipase (LIP), polyphenoloxidase (PPO), and ROS scavenging related genes glutathione S-transferase (GST), glutathione reductase (GR) and superoxide dismutase (SOD) were induced in senescent stage, suggesting ROS might be involved in the flower senescence. Besides, the expression of monoterpenoid and isoflavonoid biosynthesis genes, transcription factors (WRKY, NAC, MYB and C2H2), senescence-associated gene SAG20 also were increased during flower senescence. Conclusion: In C. lutchuensis, ethylene pathway might be the key to regulate flower senescence, and ROS signal might play a role in the flower senescence.