文献类型：期刊文献

英文题名：Hydrological restoration reshapes nitrogen cycling in alpine wetlands: Contrasting denitrification and anammox responses to rewetting

作者：Li, Niu[1] Zhou, Jianqing[2] Zhang, Chengchai[2] Tang, Yaoyao[1] Wu, Ming[1] Shao, Xuexin[1] Cao, Peipei[1] Zhang, Youzheng[3] Zhang, Long[1]

第一作者：李妞

通信作者：Zhang, L[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Wetland Conservat & Restorat, Wetland Ecosyst Res Stn Hangzhou Bay, Hangzhou 311400, Peoples R China;[2]Jingning She Autonomous Cty Ecol Forestry Dev Ctr, Hangzhou, Peoples R China;[3]Minist Nat Resources, Inst Oceanog 2, Key Lab Engn Oceanog, Key Lab Nearshore Engn Environm & Ecol Secur Zheji, Hangzhou 310012, Peoples R China

年份：2026

卷号：223

外文期刊名：ECOLOGICAL ENGINEERING

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001613396400001)】；

基金：This work was funded by Pioneer and Leading Goose R & D Program of Zhejiang (grant nos. 2025C02050, 2025C02230, 2024C02002) ; Fin-damental Research Funds of CAF (grant no. CAFYBB2024MA029) ; the Zhejiang Provincial Science and Technology Program (grant no. 2023C03120) ; National Key Research and Development Program of China (grant no. 2023YFE0101700) ; the Zhejiang Province Common-wealth Projects (grant nos. LQ23C030003 and LTGS24C160001) ; the Cooperation of Zhejiang Province and the Chinese Academy of Forestry (grant no. 2023SY11) .

语种：英文

外文关键词：Alpine wetlands; Nitrogen cycling; Hydrological restoration; Denitrification; Anammox; Microbial communities

摘要：Alpine wetlands serve as critical nitrogen (N) sinks, yet their stability is threatened by intensified drying-rewetting cycles under climate change. How hydrological restoration modulates coupled N loss pathways in these spatially heterogeneous systems remains unclear. In a before-after restoration experiment, we combined 15N-tracer incubations to quantify shifts in denitrification and anaerobic ammonium oxidation (anammox) across distinct hydrological zones. Rewetting sharply suppressed denitrification-reducing rates in upstream soils by up to 96 %-but enhanced anammox activity by up to 2.3-fold, with the strongest responses in hydrologically exposed upstream and downstream zones. These contrasting pathways were driven by zone-specific changes in soil moisture, salinity, and NH4+ availability, alongside pronounced microbial restructuring, including increased co-occurrence network complexity and shifts in keystone taxa. Structural equation models revealed that denitrification was positively regulated by salinity and microbial composition, whereas anammox was promoted by NH4+ and TC. Our results show that hydrological restoration selectively reconfigures N loss processes, favoring greenhouse gas-free anammox to sustain N removal under hydrological instability. This provides a mechanistic framework for designing zone-specific strategieses to enhance alpine wetland resilience.