文献类型：期刊文献

中文题名：大兴安岭森林沼泽类型与火干扰对土壤微生物群落影响

英文题名：The Effect of Forest Marsh and Fire Disturbance on Soil Microbial in Greater Xing'an Mountain

作者：林英华[1] 卢萍[1] 赵鲁安[2] 谭飞[3] 徐演鹏[1] 贾旭东[1] 李慧仁[4] 刘学爽[4] 韦昌雷[4] 王立中[4]

第一作者：林英华

机构：[1]中国林业科学研究院湿地研究所,北京100091;[2]黑龙江省林业监测规划院,黑龙江哈尔滨150080;[3]广西九万山国家级自然保护区,广西柳州545300;[4]大兴安岭林业集团公司农林科学研究院,黑龙江加格达奇165000

年份：2016

卷号：29

期号：1

起止页码：93-102

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：科技部(林业)公益性行业科研专项(编号:201004074);国家自然科学基金项目(31372184)资助

语种：中文

中文关键词：磷脂脂肪酸法(PLFAs);BIOLOG生态板;土壤微生物群落水平生理图谱;土壤性质;典型判别分析

外文关键词：PLFA profiles; BIOLOG Eco-Plate; community level physiological profiling; soil property; discriminant function analysis

分类号：S714.3

摘要：[目的]研究森林沼泽演替与火干扰条件下土壤微生物结构与多样性变化,为进一步揭示土壤微生物群落在森林沼泽保护与恢复中的作用提供依据。[方法]采用磷脂脂肪酸法与BIOLOG方法,研究大兴安岭南瓮河国家自然保护区内主要森林沼泽类型(兴安落叶松-狭叶杜香-藓类沼泽、兴安落叶松-兴安杜鹃-藓类沼泽、兴安落叶松+白桦-苔草沼泽)与2006年受不同火强度干扰沼泽(重度火烧的兴安落叶松-兴安杜鹃-藓类沼泽和中度火烧的兴安落叶松+白桦-苔草沼泽)土壤微生物群落特征,探讨沼泽主要发育阶段与火干扰强度对土壤微生物群落的影响。[结果]研究区域土壤微生物群落以16:00(16.29±5.62 nmol·g-1)、甲烷氧化菌(18:1ω8t)(9.89±8.61 nmol·g-1)与16:1ω7c(9.79±3.24 nmol·g-1)的微生物为优势种群。土壤微生物总PLFAs含量、革兰氏阳性菌(G+)中a15:0、i16:0、i17:0、革兰氏阴性菌(G-)中的cy19:0、真菌中的18:2ω6c、甲烷氧化菌(18:1ω8t)与森林沼泽发育阶段、火干扰明显相关(p<0.05)。一般饱和直链脂肪酸/单烯饱和脂肪酸比(Sat/Mon)偏低,其比值随沼泽发育呈现增加趋势,受到火干扰后明显增加(p<0.05);真菌/细菌比(F/B)与革兰氏阳性菌/革兰氏阴性菌(G+/G-)未随着沼泽发育呈现出规律性变化,其比值受火干扰后明显发生改变(p<0.05)。土壤细菌与真菌对6类碳源的利用能力明显不同(p<0.001),其中土壤细菌对α-D-Lactose与L-Threonine利用存在差异性(Fα-D-Lactose=2.87 p=0.080,FL-Threonine=3.00 p=0.078),土壤真菌对D-Mannitol、D-glucosaminic Acid利用存在差异性(FTween 80=2.75,p=0.088,FD-Mannitol=3.53 p=0.047,FD-glucosaminic Acid=4.67 p=0.022),但沼泽类型与火干扰未对土壤微生物功能多样性产生影响(p>0.05)。[结论]土壤微生物量与沼泽发育阶段相关;沼泽发育与火干扰改变土壤微生物群落结构。土壤细菌与真菌对碳源利用方面具有选择性。
[Objective]The aim of this paper was studied the characteristic of soil microbiological community and its diversity at forest marsh development and fire disturbance,to understand the role of soil microbial community in theforest marsh conservation and restoration. [Methods]We used phospholipid fatty acids（ PLFAs） to portrait the community composition and community level physiological profiles（ CLPP） to describe the functional diversity of the microbial community. [Results]Total of 5 types of marsh were chosen in Natural reserve area of Nanwenhe in Greater xing＇an Mountain,which included xing＇ an Larch-Ledum palustre-moss marsh,xing＇ an Larch-xing＇ an azaleamoss marsh,xing＇ an Larch-birch-carex marsh,and Larch-xing＇ an azalea-moss marsh by severe fire disturbance,xing＇an Larch-birch-carex marsh by mild fire disturbance at 2006. It was found that the PLFAs of 16： 00（ 16. 29 ±5. 62 nmol·g- 1）,18： 1ω8t（ 9. 89 ± 8. 61 nmol·g- 1） and 16： 1ω7c（ 9. 79 ± 3. 24 nmol·g- 1） were dominance in microbial community,and the dominant species contributed significantly to variations in soil microbial biomass,especially Gram positive bacteria（ cy19： 0）,Fungi（ 18： 2ω6c） and Methane oxidizing bacteria（ 18： 1ω8t）. The rate of normal saturated fatty acid and monounsaturated fatty acid increased,and significantly increased after the interference of fire（ P〈0. 05）; however,the ratio of fungi to bacteria,the rate of Gram positive bacteria and Gram negative bacteria had not changed regularly,while obviously changed after the fire disturbance. In this study,the substrate utilization pattern was significantly different between soil bacteria and soil fungi（ P〈0. 001）,of which both α-DLactose and L-Threonine was difference by utilization of soil bacteria（ Fα-D-Lactose= 2. 87 p = 0. 080,FL-Threonine= 3. 00 p = 0. 078）,and all of Tween 80,D-Mannitol,D-glucosaminic Acid were significantly difference by utilization of soil fungi（ FTween 80= 2. 75 p = 0. 088,FD-Mannitol= 3. 53 p = 0. 047,FD-glucosaminic Acid= 4. 67 p = 0. 022）,but the functional diversity of microbial community had not been effected by both the type of marsh and fire disturbance. [Conclusion]soil microbial biomass was correlated with the development stage of the marsh,and the soil microbial community structure was changed with the development stage of the marsh and fire disturbance. Soil bacteria and fungi were selective in the use of carbon resource.