文献类型：期刊文献

中文题名：花吊丝竹组培快繁育苗技术研究

英文题名：Study on Rapid Propagation Technology of Dendrocalamus minor var. amoenus

作者：张玮[1] 黄树燕[2] 谢锦忠[1] 李树忠[2] 吴继林[3]

第一作者：张玮

机构：[1]中国林业科学研究院亚热带林业研究所;[2]福建省永安林业股份有限公司种苗中心;[3]福建省永安市林业局

年份：2010

卷号：23

期号：6

起止页码：914-919

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家林业局"948"技术引进项目"竹子微繁殖技术引进"(2006-4-57);科技部农业科技成果转化资金项目"优良丛生竹种组培快繁技术转化与示范"(2010GB24320622)

语种：中文

中文关键词：花吊丝竹;组织培养;快繁;育苗

外文关键词：Dendrocalamus minor var.amoenus; tissue culture; rapid propagation; seedling breeding

分类号：S722.3

摘要：The rapid propagation technology of Dendrocalamus minor var.amoenus was studied by investigating the effects of some factors such as selection of explant,phytohormone,culture method etc.The results show that: The best month for explant collection of D.minor var.amoenus is May and June.The best position for explant collection is middle-upper part knot of semi-lignification branch.The clump shoot could be induced in medium with 3/4MS+BA 4 mg·L-1+KT 1 mg·L-1+CW 100 mL·L-1.The optimal medium for subculture of D.minor var.amoenus is 3/4MS+BA 2 mg·L-1+KT 1 mg·L-1+CW 100 mL·L-1.Liquid medium is beneficial for improving growth condition and proliferation rate of clump shoot.The medium 1/5MS+IBA 8 mg·L-1+ NAA 4.5 mg·L-1 + KT 0.1mg·L-1 is a relative suitable rooting medium for D.minor var.amoenus,with the rooting method of synchronized treatment first and then rooting.The survival rate of seedlings could be higher than 90% in substrate of fine river sand∶peat soil=3∶1.
The rapid propagation technology of Dendrocalamus minor var.amoenus was studied by investigating the effects of some factors such as selection of explant,phytohormone,culture method etc.The results show that： The best month for explant collection of D.minor var.amoenus is May and June.The best position for explant collection is middle-upper part knot of semi-lignification branch.The clump shoot could be induced in medium with 3/4MS＋BA 4 mg·L-1＋KT 1 mg·L-1＋CW 100 mL·L-1.The optimal medium for subculture of D.minor var.amoenus is 3/4MS＋BA 2 mg·L-1＋KT 1 mg·L-1＋CW 100 mL·L-1.Liquid medium is beneficial for improving growth condition and proliferation rate of clump shoot.The medium 1/5MS＋IBA 8 mg·L-1＋ NAA 4.5 mg·L-1 ＋ KT 0.1mg·L-1 is a relative suitable rooting medium for D.minor var.amoenus,with the rooting method of synchronized treatment first and then rooting.The survival rate of seedlings could be higher than 90% in substrate of fine river sand∶peat soil=3∶1.