文献类型：期刊文献

英文题名：Deciphering Genetic Architecture of Adventitious Root and Related Shoot Traits in Populus Using QTL Mapping and RNA-Seq Data

作者：Sun, Pei[1] Jia, Huixia[1] Zhang, Yahong[1] Li, Jianbo[2] Lu, Mengzhu[1,3] Hu, Jianjun[1,3]

通信作者：Hu, JJ[1];Hu, JJ[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry &, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 102300, Peoples R China;[3]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, Nanjing 210037, Peoples R China

年份：2019

卷号：20

期号：24

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000506840100009)】；

基金：This work was supported by the National Natural Science Foundation of China (31570669), the National Nonprofit Institute Research Grant of CAF (CAFYBB2017ZY008, CAFYBB2018ZY001-9), the National Key Research and Development Program of China (2017YFD0600201), and the National Key Program on Transgenic Research (2018ZX08020002).

语种：英文

外文关键词：Populus; adventitious root trait; shoot trait; QTL; RNA-Seq

摘要：Understanding the genetic architecture of adventitious root and related shoot traits will facilitate the cultivation of superior genotypes. In this study, we measured 12 adventitious root and related shoot traits of 434 F-1 genotypes originating from Populus deltoides 'Danhong' x Populus simonii 'Tongliao1' and conducted an integrative analysis of quantitative trait locus (QTL) mapping and RNA-Seq data to dissect their genetic architecture and regulatory genes. Extensive segregation, high repeatability, and significant correlation relationship were detected for the investigated traits. A total of 150 QTLs were associated with adventitious root traits, explaining 3.1-6.1% of phenotypic variation (PVE); while 83 QTLs were associated with shoot traits, explaining 3.1-19.8% of PVE. Twenty-five QTL clusters and 40 QTL hotspots were identified for the investigated traits. Ten QTL clusters were overlapped in both adventitious root traits and related shoot traits. Transcriptome analysis identified 10,172 differentially expressed genes (DEGs) among two parents, three fine rooting and three poor-rooting genotypes, 143 of which were physically located within the QTL intervals. K-means cluster and weighted gene co-expression network analysis showed that PtAAAP19 (Potri.004G111400) encoding amino acid transport protein was tightly associated with adventitious roots and highly expressed in fine-rooting genotypes. Compare with 'Danhong', 153 bp deletion in the coding sequence of PtAAAP19 in 'Tongliao1' gave rise to lack one transmembrane domain, which might cause the variation of adventitious roots. Taken together, this study deciphered the genetic basis of adventitious root and related shoot traits and provided potential function genes for genetic improvement of poplar breeding.