文献类型：期刊文献

英文题名：PcNRAMP1 Enhances Cadmium Uptake and Accumulation in Populus x canescens

作者：Yu, Wenjian[1] Deng, Shurong[1] Chen, Xin[1] Cheng, Yao[1] Li, Zhuorong[1] Wu, Jiangting[1] Zhu, Dongyue[1] Zhou, Jing[1] Cao, Yuan[1] Fayyaz, Payam[2] Shi, Wenguang[1] Luo, Zhibin[1]

通信作者：Shi, WG[1];Luo, ZB[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Silviculture Natl Forestry & Grassland Ad, Beijing 100091, Peoples R China;[2]Univ Yasuj, Agr & Nat Resources Fac, Forest Range & Watershed Management Dept, Yasuj 7591963179, Iran

年份：2022

卷号：23

期号：14

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000833326600001)】；

基金：This research was funded by the Fundamental Research Funds for the Central Non-profit Research Institutions of CAF (grant no. CAFYBB2019ZA001) and the National Natural Science Foundation of China (grant no. 31670609).

语种：英文

外文关键词：poplar; cadmium; NRAMP; Cd2+ flux; conserved amino acid residues

摘要：Poplars are proposed for the phytoremediation of heavy metal (HM) polluted soil. Characterization of genes involved in HM uptake and accumulation in poplars is crucial for improving the phytoremediation efficiency. Here, Natural Resistance-Associated Macrophage Protein 1 (NRAMP1) encoding a transporter involved in cadmium (Cd) uptake and transport was functionally characterized in Populus x canescens. Eight putative PcNRAMPs were identified in the poplar genome and most of them were primarily expressed in the roots. The expression of PcNRAMP1 was induced in Cd-exposed roots and it encoded a plasma membrane-localized protein. PcNRAMP1 showed transport activity for Cd2+ when expressed in yeast. The PcNRAMP1-overexpressed poplars enhanced net Cd2+ influxes by 39-52% in the roots and Cd accumulation by 25-29% in aerial parts compared to the wildtype (WT). However, Cd-induced biomass decreases were similar between the transgenics and WT. Further analysis displayed that the two amino acid residues of PcNRAMP1, i.e., M236 and P405, play pivotal roles in regulating its transport activity for Cd2+. These results suggest that PcNRAMP1 is a plasma membrane-localized transporter involved in Cd uptake and transporting Cd from the roots to aerial tissues, and that the conserved residues in PcNRAMP1 are essential for its Cd transport activity in poplars.