文献类型：期刊文献

英文题名：COMPARATIVE ANALYSIS OF TWO DNA EXTRACTION PROTOCOLS FROM FRESH AND DRIED WOOD OF CUNNINGHAMIA LANCEOLATA (TAXODIACEAE)

作者：Jiao, Lichao[1] Yin, Yafang[1] Xiao, Fuming[2] Sun, Qingpeng[3] Song, Kunlin[1] Jiang, Xiaomei[1]

第一作者：焦立超

通信作者：Yin, YF[1]

机构：[1]Chinese Acad Forestry, Chinese Res Inst Wood Ind, Wood Anat & Utilizat Dept, CN-100091 Beijing, Peoples R China;[2]Jiangxi Acad Forestry, CN-330032 Nanchang, Jiangxi Provinc, Peoples R China;[3]Beijing Univ Agr, Coll Biotechnol, CN-102206 Beijing, Peoples R China

年份：2012

卷号：33

期号：4

起止页码：441-456

外文期刊名：IAWA JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000313465500008)】；

基金：This work was supported financially by a project in a Chinese State Forestry Administration Project (201104058) and a Chinese National Natural Science Foundation Project (No. 30972303). We would like to express our gratitude for the technical help given by Professor Mrs. M. Xu and L. Liu at the Research Institute of Wood Industry, the Chinese Academy of Forestry.

语种：英文

外文关键词：Wood identification; DNA extraction protocols; comparison; dried wood; chloroplasts; nuclei; amyloplasts; Cunninghamia lanceolata

摘要：DNA was isolated from the sapwood, transition wood and heartwood of fresh and dried Cunninghamia lanceolata wood using two DNA extraction protocols: the modified CTAB method and the modified Qiagen kit. Our major objective was to (i) determine an optimized method for retrieving good quality and sufficient quantity of DNA from wood, and to (ii) investigate the effect of different radial positions of fresh and dried wood for DNA extraction. In comparison with the modified CTAB method, a greater quantity of higher quality DNA - both chloroplast and nuclear ribosomal DNA - was retrieved using the Qiagen kit protocol. The chloroplast DNA regions retrieved from both fresh and dried wood were successfully amplified using both protocols, but the PCR amplification for the rDNA-ITS region from the heartwood failed using both protocols. The quantity and purity of the DNA from the sapwood and transition wood (derived from nuclei and plastids in the parenchyma cells) was greater than that from the heartwood (derived mainly from amyloplasts). Due to the influence of the drying treatment, the quantity of DNA decreased by more than 50%. The optimized radial position for DNA extraction in the stem was demonstrated based on anatomical observation.