文献类型：期刊文献

中文题名：巨桉AGO基因家族的生物信息学分析

英文题名：Bioinformatics Analysis of AGO Gene Family in Eucalyptus grandis Genome

作者：范春节[1] 闫慧芳[1] 裘珍飞[1] 曾炳山[1] 刘英[1] 李湘阳[1] 王象军[1] 郭光生[1]

第一作者：范春节

机构：[1]中国林业科学研究院热带林业研究所

年份：2015

卷号：23

期号：4

起止页码：361-369

中文期刊名：热带亚热带植物学报

外文期刊名：Journal of Tropical and Subtropical Botany

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家"863"高技术研究发展计划(2013AA102705);国家自然科学基金项目(31400554);中央级公益性科研院所基本科研业务费专项(RITFYWZX201304)资助

语种：中文

中文关键词：桉树;AGO蛋白;基因家族;生物信息

外文关键词：Eucalyptus;;AGO protein;;Gene family;;Bioinformation

分类号：Q811.4

摘要：为了解巨桉（Eucalyptus grandis）中Argonaute （AGO）的功能,经全基因组序列分析,巨桉中存在14 个AGO 基因家族成员,基因长度为2676-3225 bp,编码的蛋白质具有保守的Piwi、PAZ、DUF1785 结构域.巨桉EgrAGOs 基因可分为3 组,内含子和外显子结构具有明显的组织特异性.组内成员核苷酸序列和氨基酸序列保守性较高,同源性分别达到88.14% 和82.97%.EgrAGO 基因分布于第2、4、7、8、10、11 条染色体上,在进化过程中存在着串联复制和大片段基因复制机制.预测巨桉的大部分AGO 蛋白定位于细胞核和胞质中,表现出偏碱性和亲水性,具有较高的脂溶指数.基因表达分析表明,桉树EgrAGO成员在不同组织中的表达有明显差异,与木材形成相关的组织/ 器官中有较高的表达.这些为深入研究EgrAGO 基因的功能奠定了基础.
In order to understand the function of Argonaute (AGO) in Eucalyptus grandis, the phylogeny, gene structure and expression pattern of EgrAGO were analyzed in genome-wide. The results showed that there were 14 EgrAGOs in E. grandis, which length ranged from 2676 to 3225 bp and EgrAGOs contain Piwi, PAZ and DUF1785 domains. EgrAGO genes could be divided into 3 clades, and intron and exon structures had obvious tissue specificity. The nucleotide sequence of EgrAGO and encoding amino acid sequence in each clade had homologies for 88.14%and 82.97%, respectively. Meanwhile, EgrAGO genes distribute in 2, 4, 7, 8, 10 and 11 chromosomes with tandem duplication and segmental duplication in the process of evolution. Most of EgrAGO proteins localize in nucleus and cytoplasm, showing basicity, hydrophilia and high aliphatic index. Furthermore, the expression of EgrAGOs in different tissues had signiifcant differences, and which was high in tissues or organs related with wood forming. So, these would lay a fundation for studying EgrAGO genes function in future.