文献类型：期刊文献

中文题名：干旱-溃疡病菌胁迫下北京杨水通道蛋白基因表达特征分析

英文题名：Expression pattern of aquaporin genes under drought-Botryosphaeria dothideainteraction in Populus beijingensis

作者：刘文鑫[1,2] 马长明[1] 赵嘉平[2] 万贤崇[2]

第一作者：刘文鑫

机构：[1]河北农业大学林学院;[2]中国林业科学研究院林业新技术研究所/林木遗传育种国家重点开放试验室

年份：2015

卷号：38

期号：2

起止页码：19-24

中文期刊名：河北农业大学学报

外文期刊名：Journal of Agricultural University of Hebei

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：中央级公益性科研院所基本科研业务费专项资金(CAFINT2013C15)资助

语种：中文

中文关键词：北京杨;干旱胁迫;溃疡病菌;内参基因;RT-qPCR;水通道蛋白基因

外文关键词：poplar drought canker disease reference gene RT-qPCR aquaporin

分类号：S792.11

摘要：为揭示杨树溃疡病发生进程中的水分生理学机制,本研究以1年生北京杨为材料,对干旱-溃疡病菌胁迫下的杨树管家基因表达稳定性、与植物水分运输密切相关的杨树水通道蛋白基因家族的表达特征进行了实时定量PCR(RT-qPCR)分析。结果表明:12个管家基因的表达稳定性不同,TUB、UBQ、EIF4β-L、CYP、EF1α2表达稳定性最高,ACT2和ACT11稳定性最差;配对分析显示TUB、EIF4β-L和UBQ为干旱胁迫-溃疡病菌胁迫下基因表达检测的最优内参基因组合;干旱及溃疡病菌胁迫下,水通道蛋白基因的表达模式并不相同,说明这两种胁迫对杨树水分代谢具有不同的影响;干旱-溃疡病菌双重作用下,PIP1;1、PIP1;3、PIP1;5、PIP2;4、PIP2;7等5个基因的表达显著高于干旱、溃疡病菌两种胁迫的单独作用,揭示这两种环境胁迫对水通道蛋白基因表达具有一定的协同作用。
In order to reveal the water transportation mechanism of poplar trees infected by Botryosphaeria dothidea, the expression pattern analysis of 12 poplar housekeeping genes and 9 poplar plasma membrane intrinsic protein （PIP） genes were detected by real-time quantita- tive reverse transcription polymerase chain reaction （RT-qPCR） in one-year-old Populus bei- jingensis cuttings. The results showed that TUB, UBQ, EIF4β-L, CYP and EFla2 were the top 5 stable reference genes under drought and （or） pathogen stresses, while ACT2 and ACT11 were the least stable genes. Pairwise variation of expression stability analysis revealed that TUB, EIF4β-L and UBQ would be the optimal internal control for the quantification of gene expression data. Because the expression patterns of poplar PIP genes response to fungal pathogen was not similar to which response to drought stress, the results implied that the wa- ter transportation mechanism of poplar tree under these two stresses were different. The ex- pression of 5 PIP genes （PIP1 1, PIP1 3, PIP1 5, PIP2;4, PIP2;7） in drought-patho- gen interaction were higher than which in drought or infected by pathogen, and this result suggested there were some synergistic effects among these two environmental stresses.