文献类型：期刊文献

英文题名：Overexpression and silent expression of CrGA20ox1 from Camellia reticulata 'Hentiangao' and its effect on morphological alterations in transgenic tobacco plants

作者：Wang, Jiangying[1,2] Wu, Bin[1,3] Li, Jiyuan[1] Yin, Hengfu[1] Fan, Zhengqi[1] Li, Xinlei[1] He, Libo[1]

第一作者：Wang, Jiangying

通信作者：Li, JY[1];Yin, HF[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou, Zhejiang, Peoples R China;[2]Lianyungang Acad Agr Sci, Flower Res Ctr, Lianyungang, Peoples R China;[3]Texas A&M Univ, Dept Hort Sci, College Stn, TX 77843 USA

年份：2018

卷号：137

期号：6

起止页码：903-911

外文期刊名：PLANT BREEDING

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000451029200010)】；

基金：This study was supported by the international Advanced Nonprofit Research Project of Chinese Academy of Forestry, Grant/Award Number: CAFYBB2016SZ001; International Advanced Technology of Forestry Science Introduction Program (948 Program), Grant/Award Number: 2014-4-16; Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding, Grant/Award Number: 2016C02056-12; International Science & Technology Cooperation Program of China, Grant/Award Number: 2016YFE0126100; The National agricultural production transformation project, Grant/Award Number: GB2013GB24320606; and Financial Grant Support Program of Lianyungang City, Grant/Award Number: QNJJ710

语种：英文

外文关键词：Camellia reticulata; Expression analysis; GA 20-oxidase; Gibberellin; Plant phenotype

摘要：Gibberellin 20-oxidase (GA20ox) is a multifunctional enzyme that is involved in the regulation of GA biosynthesis and the control of plant growth. We identified and characterized a GA20ox gene (CrGA20ox1) in Camellia reticulata 'Hentiangao'; subsequently, expression levels of CrGA20ox1 in four phenotypically different camellias were analysed using fluorescent quantitative real-time PCR (qRT-PCR). The results demonstrated that the CrGA20ox1 gene was expressed in all four camellias at various levels, indicating that the gene's expression intensity was positively associated with camellia height. In addition, phenotypic comparison among transgenic tobacco plants saw a nearly 3.6-fold increase in height of sense CrGA20ox1 lines as contrasted with wild-type tobacco. Interestingly and contrarily, the height of the transgenic plants expressing antisense CrGA20ox1 copies decreased by only around 0.5-fold of the controls' height. Endogenous hormone measurements illustrated that bioactive GA(4) predominantly increased in sense transgenic plants, whereas an obvious drop was observed in the antisense lines. Hence, overexpression of the CrGA20ox1 gene could accelerate vegetative growth; however, silent expression of the CrGA20ox1 gene impelled plants towards dwarfism.