文献类型：期刊文献

中文题名：枞酸的HPLC内标法定量分析研究

英文题名：Determination of Abietic Acid by HPLC with Internal Standard Method

作者：刘红军[1] 谭卫红[1] 郭晓昕[1] 冯国东[1] 周永红[1]

第一作者：刘红军

机构：[1]中国林业科学研究院林产化学工业研究所国家林业局林产化学工程重点开放性实验室,南京210042

年份：2010

卷号：22

期号：B08

起止页码：77-79

中文期刊名：天然产物研究与开发

外文期刊名：Natural Product Research and Development

收录：CSTPCD;;CSCD:【CSCD2011_2012】；

基金：中国林业科学院院长基金（CAFYBB2008009）,国家重大产业技术开发专项项目（发改高技[2008]2441号）,常州市科技攻关项目（CE2008075）

语种：中文

中文关键词：枞酸;苯甲酸;松香;HPLC;内标法

外文关键词：Abietie acid ; Benzoic acid; Rosin; HPLC ; Internal standard method

分类号：TQ351.471

摘要：建立了高效液相色谱-内标法测定枞酸含量的方法。以苯甲酸为内标物，ZORBAXSB-C18为分析柱；进样量：0．5μL；流动相：甲醇：水=90：10（v／v），流速0．5mL/min；检测器：紫外检测波长240nm；柱温室温。在枞酸浓度0．0101mg／L-0．1388mg／L范围内，枞酸样品与内标物苯甲酸的色谱峰面积比呈良好的线性关系：y=54．50264x＋0．12674；r=0．99919；重现性试验RSD为0．4594％（n=5）；精密度RSD为0．3222％（n=5）。平均回收率为99．85％，RSD为0．2799％（n=5）。稳定性实验，放置1、2、3d的同一测试样品，其稳定性RSD值为0．1899％（n=9）。验证了该方法准确、快速、灵敏度高、重现性好。
The determination method of abietic acid was established utilizing high performance liquid chromatography （HPLC） by taking benzoic acid as internal standard. The HPLC was conducted on ZORBAX SB-C18 （5μ） with methanoL/water = 90：10（v/v） as mobile phase. The flow rate of mobile phase was 0.5mL/min. The detective wavelength was set to 240 nm and room temperature as column temperature. The chromatography peak area ratio of standard to the inter- nal standard was very well correlated with the concentration of abietie acid in the range of 0.0101 mg/L - 0. 1388 mg/ L（y =54. 50264 x ＋0. 12674;r =0.99919 ）. The stability experiment was also investigated. The RSD of the reproduc- ibility, precision and stability test was 0. 4594% （ n = 5 ） ,0.3222% （ n = 5 ）, and 0.1899 % （ n = 9 ）, respectively. The average recovery rate for abietic acid was 99.85% and RSD was 0.2799% （ n = 5 ）. The method is simple, accurate and sensitive ,which can be used for the determination of the content of abietic acid.