文献类型：期刊文献

中文题名：乐昌含笑种群遗传多样性的研究

英文题名：Genetic Diversity of Michelia chapensis Dandy Populations

作者：姜景民[1] 滕花景[1] 袁金玲[1] 栾启福[1] 谭梓峰[1]

第一作者：姜景民

机构：[1]中国林业科学研究院亚热带林业研究所

年份：2005

卷号：18

期号：2

起止页码：109-113

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：科技部基础项目"亚热带珍稀树种种质资源收集;保存及编目"(2002DEA1000203)课题;浙江省林木良种技术支撑项目"浙江省阔叶绿化树种选育和快繁技术研究"

语种：中文

中文关键词：乐昌含笑;RAPD;遗传多样性;群体分化

外文关键词：Michelia chapensis;RAPD;genetic diversity;population differentiation

分类号：S718.46

摘要：基于随机扩增多态DNA(RAPD)方法分析了珍稀木兰科植物乐昌含笑6个种群的遗传多样性及分化程度。19条随机引物扩增出111个可分析位点,多态位点百分比为81 98%,经POPGENE分析发现,乐昌含笑的Nei's基因多样度(He)为0 3255,Shannon表型指数(I)为0 4751,与其它植物比较具有较高水平的遗传多样性。6个乐昌含笑群体间基因分化系数(Gst)值为0 2226,群体内的遗传变异大于群体间的遗传变异。按照遗传距离将乐昌含笑6个群体划分为两类:第1类有广西三江、湖南宜章、湖南双牌和湖南资兴的群体,第2类包括江西官山和湖南醴陵的群体;研究表明:第2类种群的遗传多样性指数高于第1类种群的遗传多样性指数。
Michelia chapensis ,belonging to Michelia genus in the family Magnoliaceae, is a elite species for timber production and for landscaping. In this paper,the genetic diversity were investigated using RAPD technique.The total DNA were extracted following a modification of CTAB method with some adjustments. A total of 144 individuals representing 6 natural population of M. chapensis were surveyed using 19 RAPD primers.At species level,the percentage of polymorphic bands was 81.98%,Nei's gene diversity was 0.325 5 and Shannon index was 0.475 1.AMOVA showed that 29.96% of the genetic diversity resulted from differentiation among populations. The coefficient of gene differentiation(Gst)generated by POPGENE32 was 0.222 6. The 6 natural populations of M. chapensis might be divided into 2 groups used UPGMA cluster system,and the genetic diversity of second group was higher than that of the first.