文献类型：期刊文献

英文题名：Proteome profiling reveals insights into cold-tolerant growth in sea buckthorn

作者：He, Caiyun[1] Gao, Guori[1] Zhang, Jianguo[1,2] Duan, Aiguo[1] Luo, Hongmei[3]

第一作者：何彩云

通信作者：Zhang, JG[1];Zhang, JG[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Tree Breeding & Cultivat,State Key Lab Tr, Beijing, Peoples R China;[2]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, Nanjing, Jiangsu, Peoples R China;[3]Chinese Acad Forestry, Expt Ctr Desert Forestry, Innermonglia, Peoples R China

年份：2016

卷号：14

期号：1

外文期刊名：PROTEOME SCIENCE

收录：;Scopus(收录号：2-s2.0-84995804449);WOS:【SCI-EXPANDED(收录号:WOS:000384933600001)】；

基金：This study was supported by the Special Fund for Forest Scientific Research in the Public Welfare (201504103) and National Natural Science Foundation of China (31470616).

语种：英文

外文关键词：Antioxidative systems; DIGE; Hippophae rhamnoides; Low temperature; Photosynthesis; Post-translational modification

摘要：Background: Low temperature is one of the crucial environmental factors limiting the productivity and distribution of plants. Sea buckthorn (Hippophae rhamnoides L.), a well recognized multipurpose plant species, live successfully in in cold desert regions. But their molecular mechanisms underlying cold tolerance are not well understood. Methods: Physiological and biochemical responses to low-temperature stress were studied in seedlings of sea buckthorn. Differentially expressed protein spots were analyzed using multiplexing fluorescent two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) coupled with matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry (MS), the concentration of phytohormone was measured using enzyme-linked immunosorbent assay, and a spectrophotometric assay was used to measure enzymatic reactions. Results: With the increase of cold stress intensity, the photosynthesis rate, transpiration rate, stomatal conductance in leaves and contents of abscisic acid (ABA) and indole acetic acid (IAA) in roots decreased significantly; however, water-use efficiency, ABA and zeatin riboside in leaves increased significantly, while cell membrane permeability, malondialdehyde and IAA in leaves increased at 7 d and then decreased at 14 d. DIGE and MS/MS analysis identified 32 of 39 differentially expressed protein spots under low-temperature stress, and their functions were mainly involved in metabolism, photosynthesis, signal transduction, antioxidative systems and post-translational modification. Conclusion: The changed protein abundance and corresponding physiological-biochemical response shed light on the molecular mechanisms related to cold tolerance in cold-tolerant plants and provide key candidate proteins for genetic improvement of plants.