文献类型：期刊文献

英文题名：Transcriptome and metabolome analyses revealed that partial desiccation treatment promotes somatic embryo germination of Picea asperata through cell wall remodeling mediated by transcription factor PaMYB12

作者：Ling, Juanjuan[1,2] Xia, Yan[2,3] Hu, Jiwen[1,2] Hao, Chunhui[1,2] Yan, Huiling[1,2] Pu, Ziyan[1,2] Zhu, Tianqing[1] Wang, Junhui[1,2]

第一作者：Ling, Juanjuan

通信作者：Zhu, TQ[1];Wang, JH[1];Wang, JH[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Natl Forestry & Grassland Adm, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Natl Forestry & Grassland Adm, Res Inst Forestry,Key Lab Tree Breeding &Cultivat, Beijing 100091, Peoples R China;[3]Southwest Univ, Coll Hort & Landscape Architecture, Key Lab Hort Sci Southern Mt Reg, Minist Educ, Chongqing 400715, Peoples R China

年份：2025

卷号：234

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20252918799219);Scopus(收录号：2-s2.0-105010683844);WOS:【SCI-EXPANDED(收录号:WOS:001575181300001)】；

基金：This project was supported by grants from Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFYBB2023MB041) , National Key Research and Development Program of China (2023YFD2200104 and 2023YFD2200603) , Fundamental Research Funds of SKLTGB (CAF) (YSH12408020481) . In addition, we thank Dr. Yuan Cao from the National Key Laboratory of Chinese Academy of Forestry for her excellent technical assistance with the morphology investigation (TEM observation) .

语种：英文

外文关键词：Picea asperata; Somatic embryo; Partial desiccation treatment; Cell wall remodeling; MYB transcription factor

摘要：Coniferous species are not only important timber sources but also have significant ecological value. Somatic embryogenesis is a powerful tool for large-scale propagation of conifers. Partial desiccation treatment (PDT) significantly promotes the germination rate of somatic embryos (SEs). However, the molecular mechanism behind this is far from understanding. In this study, morphological analysis of the Picea asperata SEs showed a significant remodeling of the cell wall structure in the hypocotyl region following PDT. Transcriptome and metabolome analysis were performed to identify the key regulator of cell wall modification in response to PDT. The results showed that the metabolic products of cellular components, such as sucrose, fructose, and glucose, were the main metabolites accumulated in the SEs after PDT. Transcriptional regulatory network analysis predicted PaMYB12 as a potential hub gene. Yeast-one-hybrid and bimolecular fluorescence complementation assay showed that PaMYB12 could directly bind to the promoter regions of genes associated with cell wall component metabolism and cell wall remodeling, including GOLS (MA_3384g0010), EXPA (MA_10425823g0010), XTH (MA_10429607g0010), and BGLU (MA_48585g0010). Furthermore, the transcript levels of these genes were increased/decreased by transient overexpression/repression of PaMYB12. In summary, PDT promotes cell wall remodeling to facility SE germination. PaMYB12, which was shown to regulate the expression of multiple genes involved in cell wall component metabolism and remodeling, is proposed to be the key regulator of this process. This knowledge would contribute to a deeper understanding of somatic embryo germination and potentially inform strategies for improving plant regeneration techniques.