文献类型：期刊文献

中文题名：不同产区厚朴nrDNA ITS序列分析及亲缘关系鉴定

英文题名：NrDNA ITS sequences analysis and genetic relationship identification of Magnolia of ficinalis from different geographical regions

作者：王洁[1] 杨旭[1] 杨志玲[1]

第一作者：王洁

机构：[1]中国林业科学研究院亚热带林业研究所

年份：2013

期号：1

起止页码：35-41

中文期刊名：广西植物

外文期刊名：Guihaia

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家科技部公益性林业专项(200704022)

语种：中文

中文关键词：nrDNA;ITS序列;厚朴;系统聚类;亲缘关系

外文关键词：nrDNA ITS sequences; Magnolia officinalis; hierarchical clustering; genetic relationship

分类号：Q789

摘要：以陕西、四川、浙江、重庆、福建、广西主产区的11个种源厚朴为材料,采用PCR直接测序法,测定不同产区厚朴的核糖体DNA ITS区序列,并结合GeneBank中相关植物的ITS序列(以同属近缘物种辛夷为外类群),应用Kimura-2遗传距离与NJ系统树分析法对不同产区厚朴的亲缘关系进行分析。结果表明:11个产区厚朴的ITS全序列长度介于593～600bp,其中,ITS1长度为214～217bp,G+C含量为54.42%～55.35%,ITS2长度为215～219bp,G+C含量为59.82%～60.95%;ITS序列共有36个变异位点,均出现在ITS1(7个)和ITS2(29个)序列中,虽然位点变异与部分叶形变化出现吻合,但无必然联系;序列间遗传分化距离为0.000～0.02792,11个产区厚朴构成3个分支,显示出产区间厚朴的亲缘关系,其中陕西洋县(SXYX)、陕西西乡(SXXX)、福建武夷山(FJWYS)及四川彭州(SCPZ)四个产区ITS序列完全一致。nrDNA ITS序列分析可作为厚朴不同产区亲缘关系鉴定的手段。
Magnolia officinalis of 11 main locality of growth （ sach as Shanxi, Sichuan, Zhejiang, Chongqing, Fujian and Guangxi and so on） were used in this paper to find the divergence of nrDNA ITS （internal transcribed spacer） se- quences for M. officinalis distributed in different areas and to provide references for the identification of the genetic relationship between different geographical regions. Their ITS sequences with direct sequencing were determined and their relationship was analyzed using Kimura-2 genetic distance and NJ （neighbor-joining）phylogenetic tree. Mean- while,M, liliiflora was used as the out-group. The results showed that the whole length of ITS sequences of M. officinalis was 593- 600 bp, in which the length of ITS1 and ITS2 were 214-217 bp and 215-219 bp, respective- ly. The contents of G＋C were 54. 42%-55.35% and 59, 82 %-60.95%,respectively. The length of 5.8S was 164 bp. A total of 36 variable sites （ITS1, 7;ITS2,29） were examined in ITS sequences, and 5.8S sequence was high- ly conserved. The sites of variation consistent with changes of partial leaf shape,but they had not necessary linkage.The genetic differentiation distance between sequences ranged from 0. 000 to 0. 02792. M. officinalis of 11 main producing regions constituted three branches, which indicated the genetic relationship of M. officinalis between dif- ferent producing areas,in which, Yangxian （SXYX）, Xixiang （SXXX）, Wuyishan （FJWYS） and Pengzhou （SCPZ） ITS sequences were the same. Therefore, nrDNA ITS sequence analysis eould be applied to the identification of genetic re- lationship for M. officinalis in different producing areas.