文献类型：期刊文献

中文题名：响应面优化水酶法制备“纸皮”扁桃油

英文题名：Optimization of Aqueous Enzymatic Process for“Zhipi”Almond Oil by Response Surface Methodology

作者：田荣荣[1,2] 牛童[1,2] 胡静[2] 肖勋泽[1] 许新桥[1] 王伟[1]

第一作者：田荣荣

机构：[1]林木遗传育种国家重点实验室,中国林业科学研究院林业研究所,北京100091;[2]北京城市学院生物医药学部,北京100094

年份：2020

卷号：35

期号：11

起止页码：61-66

中文期刊名：中国粮油学报

外文期刊名：Journal of the Chinese Cereals and Oils Association

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：中央级公益性科研院所基本科研业务费专项资金(CAFYBB2017ZA004-7);2019年北京高等学校高水平人才交叉培养“实培计划”毕设(科研类)项目(2019053)。

语种：中文

中文关键词：水酶法;响应面;“纸皮”扁桃油;出油率

外文关键词：aqueous enzymatic extraction;response surface analysis;“Zhipi”almond oil;oil yield

分类号：TS224

摘要：以“纸皮”扁桃为试材,筛选水酶法适宜的酶,进而在单因素(料液比、酶添加量、pH、酶解时间、酶解温度)实验基础上,利用Box-Behnken响应面优化获得最佳提取工艺。结果表明,水酶法制备“纸皮”扁桃油的最佳工艺为:酶为果胶酶和水解蛋白酶的复合酶(果胶酶活性∶水解蛋白酶活性=1∶5),酶解pH 3.81,料液比1∶5.135,酶添加量为果胶酶∶水解蛋白酶/g样品=237.5 U∶1187.6 U/g样品,酶解温度47.59℃,酶解时间3 h,出油率为60.73%。
Aqueous enzymatic extraction(AEE)of oil from“Zhipi”,the trial material,almond kernels was performed.Firstly,the suitable enzyme was screened.After that,the box Behnken response surface was used to optimize the extraction process on the basis of single factor(liquid/solid ratio,mixed enzyme amount,time,and temperature)test.The results showed that the optimal extraction conditions for“Zhipi”almond oil yield were mixed enzyme as pectinase activity:hydrolytic protease activity=1∶5,pH as 3.81,liquid/solid ratio as 1∶5.135,mixed enzyme amount as 237.5 U∶1187.6 U/g,temperature as 47.59℃.Under the optimum conditions,the experimental oil extraction yield was 60.73%.