文献类型：会议论文

英文题名：Biotechnological strategies for studying actinorhizal symbiosis in Casuarinaceae: transgenesis and beyond

作者：Froussart, Emilie[1,2] Zhong, Chonglu[3] Jiang, Qingbin[3] Bonneau, Jocelyne[1] Bogusz, Didier[1] Franche, Claudine[1]

第一作者：Froussart, Emilie

通信作者：Franche, C[1]

机构：[1]IRD, UMR DIADE IRD UM, Equipe Rhizogenese, 911 Ave Agropolis,BP 64501, F-34394 Montpellier 5, France;[2]Univ Montpellier, Equipe Biotechnol, UMR IATE, Campus La Valette Triolet,Pl Eugene Bataillon, F-34095 Montpellier 05, France;[3]Chinese Acad Forestry, Res Inst Trop Forestry, 682 Guangshan Yilu, Guangzhou 510520, Guangdong, Peoples R China

会议论文集：18th International Meeting on Frankia and Actinorhizal Plant (ACTINO)

会议日期：AUG 24-27, 2015

会议地点：Montpellier, FRANCE

语种：英文

外文关键词：Casuarina; Nitrogen fixation; Actinorhizal nodule; Agrobacterium; Genome editing

年份：2016

摘要：Since the recovery of the first transgenic plant in the early 1980s, plant transformation technologies have enabled advances in many aspects of basic plant science, including nitrogen-fixing root endosymbioses. Using the biological vectors Agrobacterium tumefaciens and A. rhizogenes, gene constructs have been successfully introduced in the actinorhizal tree species Casuarina glauca, thereby paving the way for functional analysis of the key genes involved in the symbiotic process with the actinobacterium Frankia. In recent years, not only studies of gene promoters in transgenic Casuarinaceae, but also the use of RNA interference to down-regulate genes of interest, have provided new insights into the early stages of the interaction between the root system and the actinobacterium. Opportunities offered by recent developments in genome editing technologies based on the engineered nucleases ZFNs (zinc-finger nucleases), TALENs (transcription activator-like effector nucleases) and RNA-guided CRISPR-Cas (clustered regularly interspaced short palindromic repeats-associated protein/Cas) will be briefly presented.