文献类型：期刊文献

中文题名：107杨次生木质部PAL基因的RT-PCR扩增及其鉴定

英文题名：Cloning and Identification of PAL Gene Amplified by RT-PCR from Populus×euramericana cv.“74/76” Second Xylem mRNA

作者：薛永常[1] 李金花[2] 卢孟柱[2] 张绮纹[2]

第一作者：薛永常

机构：[1]大连轻工业学院生物与食品学院;[2]中国林业科学研究院林业研究所

年份：2004

卷号：40

期号：4

起止页码：193-197

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家林业局 948项目---控制林木木质素合成酶基因的引进及其转化技术研究 ( 94-4 -2 1)

语种：中文

中文关键词：次生木质部;PAL基因;RT-PCR;鉴定;苯丙氨酸解氨酶;欧美杨107

外文关键词：Populus×euramericana cv.“74/76', Phenylalanine ammonia-lyase(PAL), RT-PCR, Vector construction

分类号：S792.11

摘要：A fragment of PAL (phenylalanine ammonia_lyase) gene was amplified by RT_PCR from poplar (Populus×euramericana cv. “74/76”) developing second xylem mRNA. It was cloned into pGEM-T Easy vector and identified by restriction enzyme, PCR amplification and sequencing. The sequence of the amplified DNA fragment was 565 base pairs. Alignment with the P. kitakamiensis PAL cDNA sequence retrieved from EMBL nucleotide acid database (accession number D30656) showed that the first 400 base pairs in both sequences were almost identical. Therefore the fragment was part of PAL gene. And both of sense and anti-sense expressional vectors were constructed.
A fragment of PAL (phenylalanine ammonia_lyase) gene was amplified by RT_PCR from poplar (Populus×euramericana cv. “74/76”) developing second xylem mRNA. It was cloned into pGEM-T Easy vector and identified by restriction enzyme, PCR amplification and sequencing. The sequence of the amplified DNA fragment was 565 base pairs. Alignment with the P. kitakamiensis PAL cDNA sequence retrieved from EMBL nucleotide acid database (accession number D30656) showed that the first 400 base pairs in both sequences were almost identical. Therefore the fragment was part of PAL gene. And both of sense and anti-sense expressional vectors were constructed.