文献类型：期刊文献

中文题名：枫香幼苗对铅胁迫的生理生化响应与元素分布

英文题名：Tolerance of Liquidambar formosana Seedlings to Pb: Physiological and Biochemical Response and Elements Distribution

作者：施翔[1] 王冬雪[1] 王树凤[1] 陈益泰[1] 饶龙兵[1] 孙海菁[1]

第一作者：施翔

机构：[1]中国林业科学研究院亚热带林业研究所浙江省林木育种技术研究重点实验室

年份：2019

卷号：32

期号：4

起止页码：88-95

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：浙江省农业(林木)新品种选育重大科技专项(2016C02056-10);中央级公益性科研院所基本科研业务费专项资金项目(RISF2014004);浙江省重点研发计划项目(2018C03047)

语种：中文

中文关键词：铅;枫香;耐性和积累;化学形态;微区分布

外文关键词：lead;Liquidambar formosana;tolerance and accumulation;chemical forms;micro-distribution

分类号：S718.43

摘要：[目的]研究木本植物枫香幼苗在不同浓度铅胁迫下的生长及元素微区分布和化学形态特征,探讨枫香铅耐受机制。[方法]利用水培法,研究枫香幼苗在不同浓度铅(0、50、100、200和400 mg·L ^-1 )胁迫下其生物量、叶绿素含量的变化及对铅的吸收与积累。采用化学试剂提取法分析铅在枫香植株体内存在的化学形态,并进一步采用同步辐射X射线荧光(SRXRF)技术研究铅以及其它元素在枫香根系的微区分布。[结果]枫香幼苗在不同浓度铅胁迫下叶片表现一定程度的毒害效应。根系生物量在铅低浓度处理(50 mg·L^-1 )时较对照组有一定程度增加,并且各处理组生物量耐性指数均大于0.90。铅胁迫下枫香幼苗叶片光合色素较对照组显著下降。短期试验表明,枫香幼苗对铅的吸收量大,且主要积累在植株根系,其转移系数均小于0.10。枫香幼苗叶片和根系中以盐酸提取态和醋酸提取态铅为主,其次为氯化钠提取态铅。SRXRF分析表明,枫香根组织中的表皮层区域铅浓度较高。[结论]短期水培试验表明,铅主要赋存在枫香幼苗根系表皮层区域,同时铅主要与磷酸盐配体和草酸盐配体相结合,这可能是枫香幼苗在不同铅浓度胁迫下具有较高耐性的原因。
[Objective] A greenhouse pot experiment was conducted to determine the characteristics of Pb micro-distribution and chemical forms in Liquidambar formosana seedlings and evaluate the implication on Pb toxicity and the plant tolerance.[Method] The response of seedlings to Pb concentrations (0, 50, 100, 200 and 400 mg·L^-1 ) in the solution was studied. the seedling growth, chlorophyll, carotenoid, Pb accumulation and translocation were assessed. Different chemical reagent extraction techniques were carried out to analyze the chemical forms of Pb. The characteristics of Pb distribution in root of L. formosana were investigated by synchrotron radiation X-ray fluorescence analysis.[Result]The results showed that Pb concentration treatments induced toxicity symptoms in seedlings’ leaves. Compared to the control, the biomass increased slightly at low concentration (50 mg·L ^-1 ), and the tolerance index values of all Pb treatments were great than 0.9. Under Pb stress conditions, the photosynthetic pigments significantly declined. During the experiment, a greater quantity of Pb was uptake and mostly retained in L. formosana roots, and the translocation factor values of all Pb treatments were lower than 0.1. Most of Pb in L. formosana tissues was stored in the cell wall with HAc-, HCl-, and NaCl-extractable form. The synchrotron radiation X-ray fluorescence analysis indicated that most of Pb was detected in the epidermal cells of roots.[Conclusion] Our results suggested that Pb deposited in the epidermal cells of roots and integration with phosphate or oxalate might be responsible for the tolerance of L. formosana under Pb stress in short period.