文献类型：期刊文献

英文题名：Root-Secreted Spermine Binds to Bacillus amyloliquefaciens SQR9 Histidine Kinase KinD and Modulates Biofilm Formation

作者：Liu, Yunpeng[1] Feng, Haichao[2] Chen, Lin[3] Zhang, Huihui[2] Dong, Xiaoyan[2] Xiong, Qin[1] Zhang, Ruifu[1,2]

第一作者：Liu, Yunpeng

通信作者：Zhang, RF[1];Zhang, RF[2]

机构：[1]Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Key Lab Microbial Resources Collect & Preservat, Minist Agr, Beijing 100081, Peoples R China;[2]Nanjing Agr Univ, Natl Engn Res Ctr Organ Based Fertilizers, Jiangsu Key Lab & Engn Ctr Solid Organ Waste Util, Nanjing 210095, Peoples R China;[3]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 102300, Peoples R China

年份：2020

卷号：33

期号：3

起止页码：423-432

外文期刊名：MOLECULAR PLANT-MICROBE INTERACTIONS

收录：;Scopus(收录号：2-s2.0-85080091507);WOS:【SCI-EXPANDED(收录号:WOS:000551075100006)】；

基金：This research was financially supported by the National Natural Science Foundation of China (31600088 and 31700548) and the China Postdoctoral Science Foundation (2016M591297).

语种：英文

外文关键词：Bacillus amyloliquefaciens; biocontrol; genetics and gene regulation; root exudates; KinD; interaction; ligands; molecular signaling

摘要：The signal molecules in root exudates that are sensed by plant growth-promoting rhizobacteria (PGPR) are critical to regulate their root colonization. Phosphorylated Spo0A is an important global transcriptional regulator that controls colonization and sporulation in Bacillus species. In this study, we found that deletion of kinD from PGPR strain Bacillus amyloliquefaciens SQR9, encoding an original phosphate donor of Spo0A, resulted in reduced biofilm formation in root exudates compared with the wild-type strain, indicating that KinD is responsible for sensing root exudates. Ligands of B. amyloliquefaciens SQR9 KinD in cucumber root exudates were determined by both the nontargeted ligand fishing method and the targeted surface plasmon resonance detection method. In total, we screened 80 compounds in root exudates for binding to KinD and found that spermine and guanosine could bind to KinD with dissociation constant values of 213 and 51 mu M, respectively. In addition, calcium L-threonate, N-acetyl-L-aspartic acid, sodium decanoic acid, and parabanic acid could also bind weakly to KinD The three-dimensional binding models were then constructed to demonstrate the interactions between the root-secreted signals and KinD It was observed that exogenous spermine reduced the wrinkles of biofilm when kinD was deleted, indicating that KinD might be involved in sensing root-secreted spermine and stabilizing biofilm in response to this negative effector. This study provided a new insight of interaction between a rhizobacterial sensor and root-secreted signals.