文献类型：期刊文献

英文题名：Genome-wide survey and expression analysis of the stress-associated protein gene family in desert poplar, Populus euphratica

作者：Jia, Huixia[1,2] Li, Jianbo[1,2] Zhang, Jin[1,2] Ren, Yiqiu[2] Hu, Jianjun[1,2] Lu, Mengzhu[1,2]

第一作者：Jia, Huixia

通信作者：Hu, JJ[1];Lu, MZ[1];Hu, JJ[2];Lu, MZ[2]

机构：[1]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Jiangsu, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, State Forestry Adm,Res Inst Forestry, Beijing 100091, Peoples R China

年份：2016

卷号：12

期号：4

外文期刊名：TREE GENETICS & GENOMES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000380682500016)】；

基金：This work was supported by the National Nonprofit Institute Research Grant of the Chinese Academy of Forestry (CAFYBB2014ZX001-4) and (TGB2013009) to JH.

语种：英文

外文关键词：Populus euphratica; Stress-associated proteins; Gene family; Expression analysis; Gene structure; Phylogenetic analysis

摘要：Stress-associated proteins (SAPs) are a novel class of zinc finger proteins that extensively participate in abiotic stress responses. To date, no overall analysis and expression profiling of SAP genes in woody plants have been reported. Populus euphratica is distributed in desert regions and is extraordinarily adaptable to abiotic stresses. Thus, it is regarded as a promising candidate for studying abiotic stress resistance mechanisms of woody plants. In this study, 18 non-redundant SAP genes were identified from the genome of P. euphratica using basic local alignment search tool algorithms and functional domain verification. Among these 18 PeuSAP genes, 15 were intronless. To investigate the evolutionary relationships of SAP genes in P. euphratica and other Salicaceae plants, phylogenetic analyses were performed. Subsequently, the expression profiles of the 18 PeuSAP genes were analyzed in different tissues and under various stresses (drought, salt, heat, cold, and abscisic acid (ABA) treatment) using quantitative real-time PCR. Tissue expression analysis indicated that PeuSAPs showed no tissue specificity. PeuSAPs were induced by multiple abiotic stresses, especially drought, salt, and heat stresses, perhaps because of abundant cis-acting heat shock elements and drought-inducible elements in the promoter regions of the PeuSAPs. Moreover, single nucleotide polymorphisms (SNPs) variant analysis revealed many synonymous and nonsynonymous SNPs in PeuSAP genes, but the zinc finger structure was conserved during evolution. These results provide an overview of the SAP gene family in P. euphratica and a reference for further functional research on PeuSAP genes.