文献类型：期刊文献

中文题名：西南桦以芽繁芽组培快繁研究

英文题名：In vitro Propagation of Betula alnoides by Shoot Proliferation

作者：刘英[1] 曾炳山[1] 裘珍飞[1] 谌红辉[2] 曾杰[1]

第一作者：刘英

机构：[1]中国林业科学研究院热带林业研究所;[2]中国林业科学研究院热带林业实验中心

年份：2003

卷号：16

期号：6

起止页码：715-719

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：十五"国家攻关项目"西南桦良种选育与高效栽培研究(2002BA515B0204)

语种：中文

中文关键词：西南桦;以芽繁芽;组织培养;快速繁殖;外植体;培养基;生根率

外文关键词：Betula alnoides;ratio of macro-elements;shoot proliferation;tissue culture

分类号：S792.15

摘要：以8个月生苗木上采集的枝条作为外植体,通过激素、大量元素配比等试验,成功地研发出一套西南桦以芽繁芽组培快繁技术体系。其主要内容包括:1 选择邻近顶芽带腋芽的茎段作为外植体,用HgCl2消毒3min效果最佳,诱导成功率达10%;2 其侧芽诱导与增殖培养宜选用低浓度(0 2mg L-1)的IBA或NAA;3 大量元素配比满足K∶Ca=1∶0 04～0 08、K∶Mg=1∶0 02～0 04、N∶P=1∶0 02～0 03三个比例关系时,外植体侧芽能正常萌发生长,并迅速进入增殖状态,40d转接1次,增殖倍数在4倍以上;4 选用NAA浓度为0 50mg L-1的0 4MS培养基进行生根诱导,生根率达97 9%。
Using branches collected from eight-month-old seedlings, a rapid propagation protocol by shoot proliferation was developed for Betula alnoides by adjusting the plant growth regulators and ratios of macro-elements. The main results were as follows: 1. Dipping in solution of 1/1 000 HgCl2 for 3 minutes was the best way for disinfection of branch explants and up to 10% branches were successfully induced to grow shoot; 2. To prevent branch explants from producing too much callus, only low concentration of auxins, for instance, 0.2 mg·L^(-1) IBA or NAA, could be supplemented; 3. Ratio of macro-elements was crucial for shoot germination and proliferation, the range of their ratios by mol concentration were K∶Ca =1∶0.04～0.08, K∶Mg=1∶0.02～0.04, N∶P=1∶0.02～0.03; 4.0.4 MS supplemented with 0.5 mg·L^(-1) NAA was suitable for rooting, the rooting rate reached 97.9%; and 5. The survival rate of outplanting in nursery was up to 95%.