文献类型：期刊文献

英文题名：Genome resequencing reveals demographic history and genetic architecture of seed salinity tolerance in Populus euphratica

作者：Jia, Huixia[1] Liu, Guangjian[2] Li, Jianbo[3] Zhang, Jin[4] Sun, Pei[1] Zhao, Shutang[1] Zhou, Xun[2] Lu, Mengzhu[1] Hu, Jianjun[1]

第一作者：Jia, Huixia

通信作者：Lu, MZ[1];Hu, JJ[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat Natl Forestry &, Beijing 100091, Peoples R China;[2]Beijing Novogene Co Ltd, Beijing 100083, Peoples R China;[3]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 102300, Peoples R China;[4]Oak Ridge Natl Lab, Biosci Div, POB 2009, Oak Ridge, TN 37831 USA

年份：2020

卷号：71

期号：14

起止页码：4308-4320

外文期刊名：JOURNAL OF EXPERIMENTAL BOTANY

收录：;Scopus(收录号：2-s2.0-85090303089);WOS:【SCI-EXPANDED(收录号:WOS:000553125400031)】；

基金：This work was financially supported by the National Nonprofit Institute Research Grant of CAF (CAFYBB2018ZY001-9,CAFYBB2017ZY008, CAFYBB2014ZX001-4 and TGB2013009), the Forestry Industry Research Special Funds for Public Welfare Projects (201404101), and the China Postdoctoral Science Foundation (2018M631625).

语种：英文

外文关键词：Demographic history; genome-wide association study; poplar (Populus euphratica); population structure; salinity tolerance; transgene; whole-genome resequencing

摘要：Populus euphratica is a dominant tree species in desert riparian forests and possesses extraordinary adaptation to salinity stress. Exploration of its genomic variation and molecular underpinning of salinity tolerance is important for elucidating population evolution and identifying stress-related genes. Here, we identify approximately 3.15 million single nucleotide polymorphisms using whole-genome resequencing. The natural populations of P. euphratica in northwest China are divided into four distinct clades that exhibit strong geographical distribution patterns. Pleistocene climatic fluctuations and tectonic deformation jointly shaped the extant genetic patterns. A seed germination rate-based salinity tolerance index was used to evaluate seed salinity tolerance of P. euphratica and a genome-wide association study was implemented. A total of 38 single nucleotide polymorphisms were associated with seed salinity tolerance and were located within or near 82 genes. Expression profiles showed that most of these genes were regulated under salt stress, revealing the genetic complexity of seed salinity tolerance. Furthermore, DEAD-box ATP-dependent RNA helicase 57 and one undescribed gene (CCG029559) were demonstrated to improve the seed salinity tolerance in transgenic Arabidopsis. These results provide new insights into the demographic history and genetic architecture of seed salinity tolerance in desert poplar.