文献类型：期刊文献

中文题名：文心兰花发育相关基因OAP3的克隆与表达分析

英文题名：Cloning and expression analysis of a flowering-related gene, OAP 3,in Oncidium

作者：徐小雁[1,2] 田敏[1] 王彩霞[1] 龙明华[2]

第一作者：徐小雁

机构：[1]中国林业科学研究院亚热带林业研究所;[2]广西大学农学院

年份：2011

卷号：28

期号：6

起止页码：900-906

中文期刊名：浙江农林大学学报

外文期刊名：Journal of Zhejiang A & F University

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

基金：国家林业局引进国际先进农业科学技术计划("948"计划)项目(2008-4-25);浙江省花卉产业创新团队项目(2009R50034)

语种：中文

中文关键词：分子生物学;文心兰;花器官形成;MADS-box基因;OAP3基因;qRT-PCR

外文关键词：molecular biology; Oncidium＇Milliongolds＇; floral initiation; MADS-box genes; OAP 3 gene; qRT-PCR

分类号：Q781;S682.31

摘要：为研究兰花MADS-box基因的表达与花器官发育之间的关系,以文心兰‘百万金币’Oncidium‘Milliongolds’为材料,通过RT-PCR(reverse transcription polymerase chain reaction)方法克隆到1个花发育相关的MADS-box基因,命名为OAP 3(GenBank登录号为GU644447)。该基因全长799 bp,编码204个氨基酸,具有典型的MADS结构域和K结构域,与拟南芥Arabidopsis thaliana等物种的AP 3-like同源基因所编码的氨基酸同源性达到50%～78%。进化树分析表明:OAP 3属于花器官发育B类基因,但该基因却不存在B类基因C-端共有的结构域。逆转录聚合酶链式扩增反应(qRT-PCR,quantitative RT-PCR)表达分析表明:从抽花开始,随花芽的生长,该基因相对表达丰度呈明显上升趋势;OAP 3在文心兰萼片、花瓣、唇瓣、雄蕊、雌蕊以及营养器官叶片和花茎中都表达。
To investigate the expression pattern of MADS-box gene during the development of the orchids flower,the floral organ identity gene OAP 3 with a MADS-box motif was cloned from an orchid plant through the RT-PCR,Oncidium＇Milliongolds＇（GenBank accession No.GU644447）.Then a sequence analysis and a phylogenetic analysis were conducted along with a quantitative real-time polymerase chain reaction（qRT-PCR）.The full length cDNA of OAP 3 was 799 bp with an open reading frame（ORF） encoding 204 amino acids.The deduced amino acids sequence contained a typical MADS domain and a K domain,and the sequence analysis indicated that OAP 3 shared 50%-78% homology in the amino acid sequence with Arabidopsis thaliana and other species of APETALA3 homologues.The phylogenetic analysis suggested that OAP 3 belonged to the B gene,but four consensus motifs identified in the C-terminal region of the B group genes were absent in the OAP 3 gene.The qRT-PCR analysis showed that OAP 3 mRNA increased with flower bud development,as well as in all four floral organs,vegetative leaves,and shoots.