文献类型：期刊文献

中文题名：多花黄精组培生根技术研究

英文题名：Study on inducing roots from Polygonatum cyrtonema plantlets

作者：周新华[1] 朱宜春[2] 桂尚上[3] 厉月桥[1] 聂国树[1] 邹璐[1] 林建萍[1] 黄维荣[1]

第一作者：周新华

机构：[1]中国林业科学研究院亚热带林业实验中心;[2]江西省新余市林业科学研究所;[3]广州市林业和园林绿化工程建设中心

年份：2015

卷号：33

期号：4

起止页码：102-105

中文期刊名：经济林研究

外文期刊名：Nonwood Forest Research

收录：CSTPCD;;北大核心:【北大核心2014】；

基金：中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2014MB007);林业科技推广项目"微生物肥料在油茶育苗及造林中的推广运用"(〔2012〕08号)

语种：中文

中文关键词：多花黄精;组织培养;生根率

外文关键词：Polygonatum cyrtonema;tissue culture;rooting rate

分类号：S604.3

摘要：为给多花黄精工厂化育苗和规模化栽培提供可行的关键技术,利用江西省分宜县大岗山山区选育的野生多花黄精无菌组培苗为试验材料,就不同基本培养基、不同种类与浓度的激素、不同碳源对其根茎不定根诱导的影响情况进行了试验。结果表明:最适基本培养基为1/2MS培养基;诱导生根的最佳生长素种类为NAA,其最适浓度为1.0 mg·L^(-1);最适碳源为蔗糖;最佳不定根诱导培养基为1/2MS+NAA 1.0 mg·L^(-1)+蔗糖30 g·L^(-1),其生根率为98.2%,单个组培苗的平均生根数为24.8条。
In order to provide some feasible techniques for industrialized breeding and scale cultivation ofPolygonatum cyrtonema, taking the aseptic tissue culture seedlings in wildP. cyrtonema as research objects, which gathered from Dagangshan Mountains at Fenyi County of Jiangxi Province, effects of different basic media, different kinds and concentrations of hormones and different carbon sources on adventitious root induction were tested. The result showed that the most suitable basic medium was 1/2MS; the best rooting auxin was 1.0 mg·L-1 NAA;the optimum carbon source was sucrose; the best adventitious root induction medium was 1/2MS＋NAA 1.0 mg·L-1＋sucrose 30 g·L-1, rooting rate was 98.2%, and the average rooting number per plantlet was 24.8.