文献类型：期刊文献

英文题名：Comprehensive analysis of the multi-rings mitochondrial genome of Populus tomentosa

作者：Gao, Kai[1,2] Guo, Ting[2,3] An, Xinmin[2]

第一作者：高凯;Gao, Kai

通信作者：An, XM[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China;[2]Beijing Forestry Univ, Coll Biol Sci & Technol, Natl Engn Res Ctr Tree Breeding & Ecol Restorat, Beijing Adv Innovat Ctr Tree Breeding Mol Design,S, Beijing 100083, Peoples R China;[3]Hangzhou Acad Forestry & Wetland, Hangzhou 310020, Zhejiang, Peoples R China

年份：2025

卷号：26

期号：1

外文期刊名：BMC GENOMICS

收录：;Scopus(收录号：2-s2.0-85214932288);WOS:【SCI-EXPANDED(收录号:WOS:001393543200001)】；

基金：This research was supported by the China National Key R&D Program (2021YFD2200101), Major Project of Agricultural Biological Breeding (2022ZD0401503), National Natural Science Foundation of China (31570661).

语种：英文

外文关键词：Populus tomentosa; Mitochondrial genome; Genomic structure; PacBio sequel

摘要：BackgroundPopulus tomentosa, known as Chinese white poplar, is indigenous and distributed across large areas of China, where it plays multiple important roles in forestry, agriculture, conservation, and urban horticulture. However, limited accessibility to the mitochondrial (mt) genome of P. tomentosa impedes phylogenetic and population genetic analyses and restricts functional gene research in Salicaceae family.ResultsSingle-molecule real-time (SMRT) sequencing technology was used to sequence, assemble, and annotate the mt genome of P. tomentosa. This genome has a complex structure composed of four circular molecules ranging from 153,004 to 330,873 base pairs (bp). Each of these four circular molecules contains unique gene sequences that constitute the mt genome of P. tomentosa. The mt genome comprises 69 functional genes, including 38 protein-coding genes (PCGs), 26 tRNA genes, and 5 rRNA genes. After removing duplications, 19 different tRNA coding genes remain, though only 10 amino acids can be recognized. The noncoding region constitutes 93.38% of the mt genome, comprising a large number of repetitive sequences, gene spacer regions, and insertion from chloroplast sequences. Specifically, 40 chloroplast-derived sequences, with a total length of 24,381 bp, were identified in P. tomentosa.ConclusionsIn the current study, the results provide mitochondrial genomic evidence for the maternal origin of P. tomentosa and enhance understanding of the gene dialog between organelle genomes, contributing to the conservation and utilization of the genetic resources of P. tomentosa.