文献类型：期刊文献

英文题名：An ABA-flavonoid relationship contributes to the differences in drought resistance between different sea buckthorn subspecies

作者：Gao, Guori[1,2] Lv, Zhongrui[1,2] Zhang, Guoyun[1,2] Li, Jiayi[1,2] Zhang, Jianguo[1,2,3] He, Caiyun[1,2]

第一作者：Gao, Guori

通信作者：Zhang, JG[1];He, CY[1];Zhang, JG[2];He, CY[2];Zhang, JG[3]|[a0005b245645e47e67b16]何彩云;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Natl Forestry & Grassland Adm, Res Inst Forestry, Xiangshan Rd, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm, Res Inst Forestry, Xiangshan Rd, Beijing 100091, Peoples R China;[3]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, 159 Longpan Rd, Nanjing 210037, Peoples R China

年份：2021

卷号：41

期号：5

起止页码：744-755

外文期刊名：TREE PHYSIOLOGY

收录：;Scopus(收录号：2-s2.0-85107082846);WOS:【SCI-EXPANDED(收录号:WOS:000656143200006)】；

基金：This work was supported by the Beijing Natural Science Foundation (No. 6192027).

语种：英文

外文关键词：abscisic acid; desiccation; differentially expressed genes; flavonoid; sea buckthorn

摘要：Drought is the most severe abiotic stress and hinders the normal growth and development of plants. Sea buckthorn (Hippophae rhamnoides Linn.) is a typical drought-resistant tree species. In this study, the leaves of the H. rhamnoides ssp. sinensis ('FN') and H. rhamnoides ssp. mongolica ('XY') were selected during drought-recovery cycles for RNA sequencing, and physiological and biochemical analyses. The results revealed that drought stress significantly decreased leaf water potential, net photosynthetic rate and stomatal conductance in both sea buckthorn subspecies. Similarly, the contents of flavone, flavonol, isoflavone and flavanone significantly decreased under drought stress in 'XY'. Conversely, in 'FN', the flavone and abscisic acid (ABA) contents were significantly higher under drought stress and recovered after rehydration. Meanwhile, 4618 and 6100 differentially expressed genes (DEGs) were identified under drought stress in 'FN' and 'XY', respectively. In total, 5164 DEGs were observed in the comparison between 'FN' and 'XY' under drought stress. This was more than the 3821 and 3387 DEGs found when comparing the subspecies under control and rehydration conditions, respectively. These DEGs were mainly associated with carotenoid biosynthesis, flavonoid biosynthesis, photosynthesis and plant hormone signal transduction. Six hub DEGs (ABCG5, ABCG22, ABCG32, ABCG36, ABF2 and PYL4) were identified to respond to drought stress based on weighted gene co-expression network analysis and Basic Local Alignment Search Tool (BLAST) analysis using DroughtDB. These six DEGs were annotated to play roles in the ABA-dependent signaling pathway. Sixteen RNA sequencing results involving eight genes and similar expression patterns (12/16) were validated using quantitative real-time Polymerase Chain Reaction (PCR). The biochemical and molecular mechanisms underlying the regulation of drought responses by ABA and flavonoids in sea buckthorn were clarified. In this study, gene co-expression networks were constructed, and the results suggested that the mutual regulation of ABA and flavonoid signaling contributed to the difference in drought resistance between the different sea buckthorn subspecies.