文献类型：期刊文献

中文题名：杨树CDPK基因家族的表达分析及功能预测

英文题名：Expression and Functional Analysis of CDPK Gene Family in Populus

作者：张进[1] 李建波[1] 刘伯斌[1] 陈军[1] 卢孟柱[1]

第一作者：张进

机构：[1]林木遗传育种国家重点实验室,中国林业科学研究院林业研究所

年份：2014

卷号：27

期号：5

起止页码：604-611

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金："十二.五"863计划课题"杨树分子育种与品种创制"(2011AA100201);中国博士后科学基金面上资助(2014M550104)

语种：中文

中文关键词：杨树;CDPK;基因家族;基因表达

外文关键词：Populus ; CDPK; gene family; gene expression

分类号：S792.11

摘要：为探究CDPK基因在木材形成过程中的作用,本研究在基因组水平上对杨树CDPKs基因家族的成员进行分析,找到32个CDPKs及10个CRKs成员。对CDPK基因家族成员在杨树中表达特性进行了分析,发现其家族成员在不同组织、不同发育阶段以及毛白杨次生维管发育不同时期的表达特性不同。分析杨树CDPK基因家族中3个基因Pt CPK1、Pt CPK6、Pt CPK15,其蛋白质一级结构均存在1个跨膜区,其蛋白定位在细胞膜上。
Calcium-dependent protein kinases （CDPKs） are important Ca2^＋ sensors and effectors in Ca2^＋ signal transduction pathway. They play crucial roles in regulation of plant development and tolerance of various environ- mental stresses. In this study, a genome-wide analysis of Populus CDPKs was performed, including phylogeny, gene structure and expression patterns. Thirty-two CDPK genes and ten CRK genes were identified. Furthermore, the gene expression profiles showed that a number of Populus CDPK differentially expressed across different tissues and developmental stages. Three Populus CDPK gene, i.e. PtCPK1, PtCPK6 and PtCPK15, were cloned and ana-lyzed, revealing their proteins localized in cell membrane. These results may lay the foundation for functional analy- sis of Populus CDPK gene family in the future.