文献类型：期刊文献

英文题名：ThWRKY4 from Tamarix hispida Can Form Homodimers and Heterodimers and Is Involved in Abiotic Stress Responses

作者：Wang, Liuqiang[1] Zheng, Lei[2] Zhang, Chunrui[2] Wang, Yucheng[2] Lu, Mengzhu[1] Gao, Caiqiu[2]

第一作者：王留强

通信作者：Lu, MZ[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry A, Beijing 100091, Peoples R China;[2]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China

年份：2015

卷号：16

期号：11

起止页码：27097-27106

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000365648300077)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 31200498), the Special Fund for Outstanding Talented Person of Harbin City (2012RFXXN023) and the Fundamental Research Funds for the Central Universities (2572014AA07). We also thank the editor and reviewers for critical comments and thoughtful suggestions.

语种：英文

外文关键词：WRKY; stress response; dimerization; Tamarix hispida; yeast two-hybrid

摘要：WRKY proteins are a large family of transcription factors that are involved in diverse developmental processes and abiotic stress responses in plants. However, our knowledge of the regulatory mechanisms of WRKYs participation in protein-protein interactions is still fragmentary, and such protein-protein interactions are fundamental in understanding biological networks and the functions of proteins. In this study, we report that a WRKY protein from Tamarix hispida, ThWRKY4, can form both homodimers and heterodimers with ThWRKY2 and ThWRKY3. In addition, ThWRKY2 and ThWRKY3 can both bind to W-box motif with binding affinities similar to that of ThWRKY4. Further, the expression patterns of ThWRKY2 and ThWRKY3 are similar to that of ThWRKY4 when plants are exposed to abscisic acid (ABA). Subcellular localization shows that these three ThWRKY proteins are nuclear proteins. Taken together, these results demonstrate that ThWRKY4 is a dimeric protein that can form functional homodimers or heterodimers that are involved in abiotic stress responses.