文献类型：期刊文献

中文题名：三年桐、千年桐感染枯萎病病原菌后的生理反应

英文题名：Physiological Response of Vernicia fordi and V. montana upon Inoculation with Fusarium oxysporum

作者：杨素素[1] 高暝[1] 朱慧萍[1] 刘英冠[1] 汪阳东[1] 陈益存[1]

第一作者：杨素素

机构：[1]中国林业科学研究院亚热带林业研究所

年份：2014

卷号：27

期号：6

起止页码：752-757

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家自然科学基金青年科学基金项目(31200485)

语种：中文

中文关键词：油桐枯萎病;尖孢镰刀菌;三年桐;千年桐

外文关键词：Tung wilt ; Fusarium oxysporum ; Vernicia fordi ; Vernicia montana

分类号：S763.1

摘要：枯萎病是油桐毁灭性病害。中国广泛种植的油桐有三年桐和千年桐,三年桐易感枯萎病,千年桐抗枯萎病。为探讨三年桐与千年桐在枯萎病应答过程中的差异,在对油桐枯萎病灾区调研的基础上,进行枯萎病病原菌的分离、鉴定,并进一步探讨三年桐、千年桐接种枯萎病病原菌后超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量的变化。结果表明:油桐枯萎病病原菌为尖孢镰刀菌,接种试验显示千年桐在接种尖孢镰刀菌后SOD、POD、CAT较三年桐均呈现较高活性,在接种后SOD、POD活性先升高后降低,CAT活性升高并维持高活性,MDA含量变化不明显;三年桐SOD活性和MDA含量均先升高后降低,POD活性先降低再升高,CAT活性先降低后升高又降低。千年桐作为抗病种,其抗病性可能与其本身具有较高的SOD、POD、CAT活性有关,并且和病原菌感染后酶活上升有关;三年桐为易感病种,接种后SOD、POD、CAT活性有变化,但仍不能抵御病原菌的危害。
Tung wilt is a devastating disease for tung tree,which is regarded as the tung plague.Vernicia fordii and V.montana are two Chinese native tung species.V.fordii is susceptible to tung wilt disease while V.montana is resistant.To explore the difference of the two species responding to the pathogen,the research focused on the isolation and identification of the pathogen,and the activities of superoxide dismutase （SOD）,peroxidase （POD） and catalase （CAT） and malondialdehyde （MDA） content of the two species after inoculation with the pathogen were measured.It turned out that the tung wilt pathogen was Fusarium oxysporum.The infection experintent revealed that the activities of SOD,POD and CAT in V.montana were higher than that of V.fordii after inoculation with F.oxysporum,in V.montana,the activities of SOD and POD increased at first and then decreased,the activity of CAT increased at first and maintained at a high level,the MDA content had no significant change.In V.fordii,both SOD activity and MDA content went up at the beginning and then down,the POD activity reduced at first and then increased,the CAT activity decreased at first and then rose and decreased at last.In conclusion,the resistant V.montana owned higher level activities of SOD,POD,CAT,and showed more increasing upon infection with the pathogen F.oxysporum than that of susceptible V.fordii.