文献类型：期刊文献

中文题名：桑叶总生物碱提取及其与牛血清蛋白结合能力研究

英文题名：Extraction of Total Alkaloids From Mulberry Leaves and Its Binding With Bovine Serum Albumin

作者：汤丽华[1] 管勤昊[1] 闫波雯[1] 徐曼[1] 张亮亮[2] 胡新宇[1]

第一作者：汤丽华

机构：[1]中国林业科学研究院林产化学工业研究所,江苏省生物质能源与材料重点实验室,国家林业和草原局林产化学工程重点实验室,林木生物质低碳高效利用国家工程研究中心,江苏省林业资源高效加工利用协同创新中心,江苏南京210042;[2]华侨大学先进碳转化技术研究院,福建厦门361021

年份：2026

卷号：60

期号：1

起止页码：37-45

中文期刊名：生物质化学工程

外文期刊名：Biomass Chemical Engineering

基金：国家重点研发计划资助项目(2022YFD1300903)。

语种：中文

中文关键词：桑叶;总生物碱;牛血清蛋白;液相色谱/质谱联用;圆二色光谱

外文关键词：mulberry leaves;total alkaloids;bovine serum albmin;HPLC-UV/MS chromatography;circular dichroic spectrum

分类号：TQ35

摘要：采用超声波低温提取工艺从桑叶中提取总生物碱,在单因素试验的基础上通过响应面法优化提取工艺,利用液相色谱/质谱联用仪对桑叶总生物碱主要结构进行鉴定分析,并通过系列光谱研究了桑叶总生物碱与牛血清蛋白(BSA)的结合能力。桑叶总生物碱较佳提取工艺为桑叶3.0 g、0.05 mol/L盐酸溶液与桑叶的液料比10∶1(mL∶g)、再加入乙醇配制成乙醇体积分数33%的混合溶液,在超声波功率250 W、提取温度40℃、提取时间30 min、提取2次。在此条件下桑叶总生物碱提取量为2.596±0.13 mg/g。经液相色谱/质谱分析可知,桑叶总生物碱主要有4种结构:2-氧-α-D-半乳吡喃糖苷-脱氧野尻霉素、1-脱氧野尻霉素(1-DNJ)、1,4-二脱氧-1,4-亚氨基-D-阿拉伯糖醇与荞麦碱。由荧光光谱分析可知,桑叶总生物碱能够与牛血清蛋白结合,结合常数为(9.79±0.23)×10^(3)L/(mol·s)。圆二色光谱分析结果证实了桑叶总生物碱可以改变牛血清蛋白二级结构。
The total alkaloids were extracted from mulberry leaves by ultrasonic low-temperature extraction process.Based on single factor experiments,the extraction process was optimized by response surface method.The main structures of total alkaloids from mulberry leaves were identified and analyzed by liquid chromatography/mass spectrometry,and the binding ability of total alkaloids from mulberry leaves to bovine serum albumin(BSA)was studied through a series of spectra.The results showed that the optimal extraction process of total alkaloids from mulberry leaves was as follows:mulberry leaves of 3.0 g,solid-liquid ratio of 0.05 mol/L hydrochloric acid solution to mulberry leaves 10∶1(mL∶g),ethanol volume fraction of 33%,ultrasonic power of 250 W,extraction temperature of 40℃,extraction time of 30 min,and two times of extraction.Under these conditions,the extraction amount of total alkaloids from mulberry leaves was 2.596±0.13 mg/g.According to the analysis by liquid chromatography/mass spectrometry(LC/MS),there were four main structures of total alkaloids from mulberry leaves,2-O-α-D-galactopyranosyl-deoxynojirimycin,1-deoxynojirimycin(1-DNJ),1,4-dideoxy-1,4-imino-D-arabinitol and fagomine.It could be seen from the fluorescence spectrum that the total alkaloids from mulberry leaves could bind to BSA with a binding constant of(9.79±0.23)×10^(3) L/(mol·s).Circular dichroism analysis confirmed that total alkaloids from mulberry leaves could alter the secondary structure of BSA.