文献类型：期刊文献

中文题名：拟南芥尾翼茎突变体tfos基因的分子标记定位

英文题名：Chromosomal Location of Tail-Fins-on-Stem Gene in Arabidopsis thaliana

作者：李红英[1,2] 杨海峰[3] 赵树堂[2] 唐芳[2] 戚晓利[4] 陈军[2] 卢孟柱[2]

第一作者：李红英

机构：[1]南京林业大学林木遗传与生物技术省部共建教育部重点实验室;[2]中国林业科学研究院林业研究所林木遗传育种国家重点实验室;[3]内蒙古农业大学林学院;[4]黑龙江佳木斯大学生命科学学院

年份：2012

卷号：48

期号：6

起止页码：1-7

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金项目(31030018)

语种：中文

中文关键词：拟南芥;尾翼茎突变体;木材形成;分子标记;基因定位

外文关键词：Arabidopsis thaliana;tfos;wood formation;molecular marker;gene location

分类号：S718.46;Q943.2

摘要：在短日照生长条件下,拟南芥维管发育有一定量的次生生长,可模拟林木木材的形成过程。前期研究中,筛选到 1 个突变体,在短日照生长条件下,相对于野生型,该突变体植株矮化且茎中下部有脊状结构附着,并伴随有发育迟缓、营养生长时期延长和莲座叶叶片边缘呈锯齿状等性状,将其命名为尾翼茎突变体( tfos) 。切片显微观察表明,尾翼组织具有明显维管结构,推测为茎内部细胞不正常分化导致该表型; 遗传分析显示,突变性状受隐性单基因控制。进一步利用分子标记技术对该基因进行定位分析,结果将其定位到 1 号染色体上,与 SSLP 标记F11A17-48074 紧密连锁。
Arabidopsis thaliana undergoes to a certain degree secondary growth under short day conditions,thus it can be used to study wood formation.In our previous work,we screened a mutant(tail-fins-on-stem,tfos) that displayed unique characteristics under short day conditions,such as shorter stem with a few ridge-like structures on the middle and the basal stem,slower growth and twisted rosette leaves with serrated margin,in comparison with the wild type.Microscopic observation showed that there was vascular structure in the middle of the ridge,likely due to abnormal differentiation of the cells in the stem.Genetic analysis indicated that the mutant trait was controlled by a single recessive nuclear gene.Further analysis,with molecular markers,showed that the mutant gene was mapped on the Chromosome I and displayed co-separation with the SSLP marker F11A17-48074.This study paved a way for cloning the gene controlling the phenotypes.