文献类型：期刊文献

英文题名：Transcription factor PagMYB31 positively regulates cambium activity and negatively regulates xylem development in poplar

作者：Zhang, Yanhui[1] Chen, Song[2] Xu, Linghua[3] Chu, Shimin[4] Yan, Xiaojing[1] Lin, Lanying[4] Wen, Jialong[3] Zheng, Bo[5] Chen, Su[2] Li, Quanzi[1]

通信作者：Li, QZ[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China;[3]Beijing Forestry Univ, Beijing Key Lab Lignocellulos Chem, Beijing 100083, Peoples R China;[4]Chinese Acad Forestry, Res Inst Wood Ind, Beijing 100091, Peoples R China;[5]Huazhong Agr Univ, Coll Hort & Forestry Sci, Poplar Res Ctr, Wuhan 430070, Peoples R China

年份：2024

卷号：36

期号：5

起止页码：1806-1828

外文期刊名：PLANT CELL

收录：;Scopus(收录号：2-s2.0-85190903465);WOS:【SCI-EXPANDED(收录号:WOS:001173815500001)】；

基金：This work was supported by the National Key Research and Development Program of China (grant no. 2023YFD2200204) and the National Natural Science Foundation of China (grant no. 32071787).

语种：英文

摘要：Wood formation involves consecutive developmental steps, including cell division of vascular cambium, xylem cell expansion, secondary cell wall (SCW) deposition, and programmed cell death. In this study, we identified PagMYB31 as a coordinator regulating these processes in Populus alba x Populus glandulosa and built a PagMYB31-mediated transcriptional regulatory network. PagMYB31 mutation caused fewer layers of cambial cells, larger fusiform initials, ray initials, vessels, fiber and ray cells, and enhanced xylem cell SCW thickening, showing that PagMYB31 positively regulates cambial cell proliferation and negatively regulates xylem cell expansion and SCW biosynthesis. PagMYB31 repressed xylem cell expansion and SCW thickening through directly inhibiting wall-modifying enzyme genes and the transcription factor genes that activate the whole SCW biosynthetic program, respectively. In cambium, PagMYB31 could promote cambial activity through TRACHEARY ELEMENT DIFFERENTIATION INHIBITORY FACTOR (TDIF)/PHLOEM INTERCALATED WITH XYLEM (PXY) signaling by directly regulating CLAVATA3/ESR-RELATED (CLE) genes, and it could also directly activate WUSCHEL HOMEOBOX RELATED4 (PagWOX4), forming a feedforward regulation. We also observed that PagMYB31 could either promote cell proliferation through the MYB31-MYB72-WOX4 module or inhibit cambial activity through the MYB31-MYB72-VASCULAR CAMBIUM-RELATED MADS2 (VCM2)/PIN-FORMED5 (PIN5) modules, suggesting its role in maintaining the homeostasis of vascular cambium. PagMYB31 could be a potential target to manipulate different developmental stages of wood formation. Transcription factor MYB31 regulates cambial cell proliferation, xylem cell expansion, and secondary cell wall thickening in poplar.