文献类型：期刊文献

英文题名：Genomic Data Reveals Profound Genetic Structure and Multiple Glacial Refugia in Lonicera oblata (Caprifoliaceae), a Threatened Montane Shrub Endemic to North China

作者：Mu, Xian-Yun[1] Wu, Yuan-Mi[1] Shen, Xue-Li[1] Tong, Ling[1] Lei, Feng-Wei[1] Xia, Xiao-Fei[2] Ning, Yu[3]

第一作者：Mu, Xian-Yun

通信作者：Mu, XY[1]

机构：[1]Beijing Forestry Univ, Sch Ecol & Nat Conservat, Lab Systemat Evolut & Biogeog Woody Plants, Beijing, Peoples R China;[2]Beijing Museum Nat Hist, Beijing, Peoples R China;[3]Chinese Acad Forestry, Inst Wetland Res, Beijing, Peoples R China

年份：2022

卷号：13

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000803894900001)】；

基金：This study was supported by the Beijing Natural Science Foundation (grant no. 5192012), the National Natural Science Foundation of China (grant nos. 32070235 and 31800348), and the Plant Germplasm Investigation Program from the Ministry of Agriculture and Rural Affairs of China (grant no. 13200346).

语种：英文

外文关键词：conservation units; genetic diversity; Lonicera oblata; glacial refugia; Taihang Mountains; threatened species; Yan Mountains

摘要：Characterizing genetic diversity and structure and identifying conservation units are both crucial for the conservation and management of threatened species. The development of high-throughput sequencing technology provides exciting opportunities for conservation genetics. Here, we employed the powerful SuperGBS method to identify 33, 758 high-quality single-nucleotide polymorphisms (SNP) from 134 individuals of a critically endangered montane shrub endemic to North China, Lonicera oblata. A low level of genetic diversity and a high degree of genetic differentiation among populations were observed based on the SNP data. Both principal component and phylogenetic analyses detected seven clusters, which correspond exactly to the seven geographic populations. Under the optimal K = 7, Admixture suggested the combination of the two small and geographically neighboring populations in the Taihang Mountains, Dongling Mountains, and Lijiazhuang, while the division of the big population of Jiankou Great Wall in the Yan Mountains into two clusters. High population genetic diversity and a large number of private alleles were detected in the four large populations, while low diversity and non-private alleles were observed for the remaining three small populations, implying the importance of these large populations as conservation units in priority. Demographic history inference suggested two drastic contractions of population size events that occurred after the Middle Pleistocene Transition and the Last Glacial Maximum, respectively. Combining our previous ecological niche modeling results with the present genomic data, there was a possible presence of glacial refugia in the Taihang and Yan Mountains, North China. This study provides valuable data for the conservation and management of L. oblata and broadens the understanding of the high biodiversity in the Taihang and Yan Mountains.