文献类型：期刊文献

英文题名：Metal-organic framework promoting high-solids enzymatic hydrolysis of untreated corncob residues

作者：Zheng, Tianran[1] Yang, Luan[1] Ding, Meili[1] Huang, Chen[1,2] Yao, Jianfeng[1]

第一作者：Zheng, Tianran

通信作者：Yao, JF[1]

机构：[1]Nanjing Forestry Univ, Coll Chem Engn, Jiangsu Coinnovat Ctr Efficient Proc & Utilizat F, Jiangsu Prov Key Lab Green Biomass Based Fuels &, Nanjing 210037, Peoples R China;[2]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Nanjing 210042, Peoples R China

年份：2022

卷号：344

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20214411087444);Scopus(收录号：2-s2.0-85117938159);WOS:【SCI-EXPANDED(收录号:WOS:000719968900008)】；

基金：The authors are grateful for Jiangsu Forestry Science and Technology Innovation and Extension Project (LYKJ [2021] 04) , and China Post-doctoral Science Foundation (2019M661855) .

语种：英文

外文关键词：Metal-organic framework; Corncob residues; High-solids substrate; Low cellulase loading; Promoted enzymatic hydrolysis

摘要：Metal-organic frameworks (MOFs) could serve as efficient matrixes to immobilize cellulase because of their high stability and porous morphology. Herein, the Zr-based MOFs (UiO-66 and UiO-66-NH2) assisted 20 wt% high-solids hydrolysis of untreated corncob residues (CRs) at low enzyme loading was investigated. Glucan hydrolysis yields increased to 60.55% and 71.47% by separately adding 4 g/L UiO-66 and UiO-66-NH2 at 5 FPU/g-glucan cellulase dosage. The maximum hydrolysis yield reached 90.01% at 10 FPU/g-glucan in the presence of 4 g/L UiO-66-NH2. Analysis of free protein concentration and cellulase activity suggested that MOFs effectively increased cellulase catalytic activity and stability, thus boosted CRs enzymatic hydrolysis efficiency. Additionally, UiO-66-NH2 immobilization gave a high catalytic activity because of the abundant anchor sites of NH2 groups. This research presents the promising future of MOFs' application in lignocellulosic biomass bioconversion and other areas requiring immobilized enzymes.