文献类型：期刊文献

英文题名：High temperature and UV-C treatments affect stilbenoid accumulation and related gene expression levels in Gnetum parvifolium

作者：Deng, Nan[1] Liu, Caixia[1] Chang, Ermei[1] Ji, Jing[1] Yao, Xiamei[1] Yue, Jianyun[1] Bartish, Igor V.[2] Chen, Lanzhen[3,4] Jiang, Zeping[1] Shi, Shengqing[1]

第一作者：Deng, Nan

通信作者：Jiang, ZP[1];Shi, SQ[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Acad Sci Czech Republ, Inst Bot, Dept Genet Ecol, Prague, Czech Republic;[3]Chinese Acad Agr Sci, Inst Apicultural Res, Beijing, Peoples R China;[4]Qual & Safety Minist Agr, Risk Assessment Lab Bee Prod, Beijing, Peoples R China

年份：2017

卷号：25

起止页码：43-49

外文期刊名：ELECTRONIC JOURNAL OF BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000392435900008)】；

基金：This work was support by the Special Fund for State Key Laboratory of Tree Genetics and Breeding (TGB2013012), 948 project (2012-4-43), and Fund of National Non-profit Research Institutions of CAF (RIF2013-12).

语种：英文

外文关键词：Antibacterial; Anticancer; Antioxidative; Chinese medicine; Gnetophyta; Natural bioactive compounds; Polyphenols; Real-time PCR; Seedlings; Stilbenes; Stress-treated roots

摘要：Background: Gnetum parvifolium stems and roots have been used for a long time in traditional Chinese medicines. Stilbenes are bioactive compounds present in G. parvifolium plants, and they possess antioxidative and anticancer properties. However, little is known about the responses of G. parvifolium stilbene biosynthetic pathways to stress conditions. Therefore, we investigated stilbene biosynthesis, including the expression of relevant genes, in G. parvifolium exposed to high-temperature and ultraviolet-C treatments. Results: High temperatures did not influence the accumulation of total stilbenes in stems but decreased stilbene concentrations in roots at 3 h, with a subsequent restoration to control levels. In contrast, ultraviolet irradiation induced the accumulation of total stilbenes in stems but not in roots. We also observed that high temperatures inhibited the production of resveratrol and piceatannol in G. parvifolium stems and roots, whereas ultraviolet treatments initially inhibited their accumulation (up to 6 h) but induced their production at later time points. Analyses of specific genes (i.e., PAL, C4H, 4CL, STS, and CYP) revealed that their expression levels generally increased in stress-treated stems and roots, although there was some variability in the expression profiles during treatments. Conclusions: Our results indicated that high temperatures and ultraviolet irradiation differentially affect the biosynthesis of specific stilbenes in G. parvifolium stems and roots. Therefore, cultivating G. parvifolium seedlings under optimal stress conditions may increase the biosynthesis of specific stilbene compounds. (C) 2016 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved.