文献类型：期刊文献

中文题名：山西省酸枣遗传多样性及遗传结构分析

英文题名：Genetic Diversity and Genetic Structure of Ziziphus jujuba var.spinosa in Shanxi

作者：胡晓艳[1] 杜淑辉[1] 王兆山[2] 韩有志[1]

第一作者：胡晓艳

机构：[1]山西农业大学林学院,北方功能油料树种培育与研发山西省重点实验室,山西太谷030800;[2]中国林业科学研究院林业研究所,北京100091

年份：2020

卷号：33

期号：5

起止页码：137-144

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：山西农业大学科技创新项目(2017YJ22);山西省重点研发计划重点项目(201703D211011-2)。

语种：中文

中文关键词：酸枣;单拷贝核基因标记;遗传多样性;遗传结构;居群动态

外文关键词：Ziziphus jujuba var.spinosa;single-copy nuclear gene marker;genetic diversity;genetic structure;population demography

分类号：S718.46

摘要：[目的]山西省是我国酸枣集中分布区之一,开展山西省酸枣遗传多样性研究,为酸枣种质资源保护及可持续利用奠定基础。[方法]本研究利用生物信息学方法筛选酸枣中单拷贝核基因标记,对山西省14个酸枣居群的遗传多样性、遗传结构及居群动态进行分析。[结果]本研究筛选得到6个单拷贝核基因标记用于开展山西省酸枣种质资源相关研究。单拷贝核基因标记的长度为199~846 bp,分离位点数目和单倍型多样性平均值分别为37和0.857,表明单拷贝核基因标记多样性较高。中性检验表明:所有单拷贝核基因标记均符合中性进化假设。山西省酸枣表现出产高的遗传多样性(π=0.00720,θw=0.00925),这与高度异交及居群历史动态有关。失配分布分析表明:山西省酸枣在进化历史上经历过居群持续扩张,这与黄土高原地区受第四纪冰期循环影响小有关。STRUCTURE分析表明:山西酸枣居群没有形成明显的遗传结构。AMOVA分析显示:居群间遗传分化水平较高(Fst=0.145),居群内变异是总变异的主要来源(85.48%)。Mantel test表明:遗传距离和地理距离之间没有相关性(r=0.1775,P=0.216.)。[结论]单拷贝核基因标记可以应用于酸枣种质资源遗传学研究。山西省酸枣种质资源遗传多样性水平较高,居群间遗传分化较明显。
[Objective] To study the genetic diversity and genetic structure of Ziziphus jujuba var. spinosa in Shanxi Province so as to contribute to the conservation and sustainable utilization of Z. jujuba var. spinosa germplasm.[Method] Single-copy nuclear gene markers were screened and the genetic diversity, genetic structure and population demography of 14 Z. jujuba var. spinosa populations distributed in Shanxi were analyzed. [Result] 6 singlecopy nuclear gene markers were screened and utilized in analysis. The length of the markers varied between 199 and846 bp. The average number of segregating sites and haplotype diversity were 37 and 0.857, indicating high level of nucleotide diversity of these markers. All the markers were constrained to the neutral evolution model. The genetic diversity of Z. jujuba var. spinosa in Shanxi was relatively high(π=0.007 20, θw=0.009 25). High level of outcrossing and complex population demography resulted in high level of genetic diversity. Mismatch distribution analysis showed that Z. jujuba var. spinosa underwent population expansion in evolutionary history, which resulted from less affects by Quaternary glaciation cycles in the Loss Plateau. STRUCTURE analysis showed no genetic structure formed among populations. AMOVA analysis indicated that the populations of Z. jujuba var. spinosa in Shanxi showed moderately high level of genetic differentiation(Fst=0.145). Variation within populations contributed majority to the total variation(85.48%). Mantel test showed that no correlation was found between genetic distance and geographical distance. [Conclusion] Z. jujuba var. spinosa distributed in Shanxi shows relatively high level of genetic diversity and moderate genetic differentiation.