文献类型：期刊文献

英文题名：Metabolomic and transcriptomic analyses provide insights into the flavonoid biosynthesis in sea buckthorn (Hippophae rhamnoides L.)

作者：Song, Yating[1] Zhang, Guoyun[1] Chen, Ning[1] Zhang, Jianguo[1,2] He, Caiyun[1]

第一作者：Song, Yating

通信作者：Zhang, JG[1];He, CY[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm,Res Inst Forestry, Beijing, Peoples R China;[2]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry Southe, Nanjing, Peoples R China

年份：2023

卷号：187

外文期刊名：LWT-FOOD SCIENCE AND TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001079480400001)】；

基金：This work was supported by National Natural Science Foundation of China (32101554) and National Natural Science Foundation of China (U2003116) .

语种：英文

外文关键词：Sea buckthorn; Transcriptomic analysis; Metabolites; Flavonoid biosynthesis; HrMYB114

摘要：Sea buckthorn is a plant with similarities to both food and medicine, possessing abundant flavonoids. However, the metabolic mechanism of flavonoid synthesis in sea buckthorn is unclear. Here, we performed transcriptomic and metabolomic analyses to explore the metabolic characteristics of flavonoids in different sea buckthorn tissues and their associated gene regulatory networks. A total of 441 metabolites were identified from sea buckthorn by metabolomics (UPLC-MS/MS), which includes 104 flavonoids with significant differences in the relative contents among tissues. Transcriptomic analysis identified 75 genes involved in the flavonoid biosynthesis pathway. The weighted gene correlation network analysis revealed that the MEdarkgreen module showed a significant cor-relation with flavonoid compounds and comprised ten genes related to flavonoid synthesis. Overexpression of HrMYB114 resulted in a significant increase in flavonoid content and expression of structural genes in the flavonoid synthesis pathway in transgenic hairy roots. This study provided a comprehensive analysis of meta-bolic and transcriptomic profiles, which helped to investigate the molecular mechanism of flavonoid biosynthesis.