文献类型：期刊文献

英文题名：Differentiated responses of the phyllosphere bacterial community of the yellowhorn tree to precipitation and temperature regimes across Northern China

作者：Wang, Weixiong[1,2,3] Hu, Congcong[4] Chang, Yu[1,3] Wang, Libing[5] Bi, Quanxin[5] Lu, Xin[6,7] Zheng, Zhimin[1,2] Zheng, Xiaoqi[8] Wu, Di[1,2,3] Niu, Ben[1,2,3]

第一作者：Wang, Weixiong

通信作者：Wu, D[1];Niu, B[1];Wu, D[2];Niu, B[2];Wu, D[3];Niu, B[3];Zheng, XQ[4]

机构：[1]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin, Peoples R China;[2]Northeast Forestry Univ, Coll Forestry, Ctr Basic Forestry Res, Harbin, Peoples R China;[3]Northeast Forestry Univ, Coll Life Sci, Harbin, Peoples R China;[4]Shanghai Normal Univ, Dept Math, Shanghai, Peoples R China;[5]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[6]Chifeng Res Inst Forestry Sci, Chifeng, Peoples R China;[7]Natl Forestry & Grassland Shiny Leaved Yellowhorn, Chifeng, Peoples R China;[8]Shanghai Jiao Tong Univ, Ctr Single Cell Omics, Sch Publ Hlth, Sch Med, Shanghai, Peoples R China

年份：2023

卷号：14

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;Scopus(收录号：2-s2.0-85183616097);WOS:【SCI-EXPANDED(收录号:WOS:001097612900001)】；

基金：We thank members of the BN laboratory for valuable advice.r The author(s) declare financial support was received for the research, authorship, and/or publication of this article. Start-up Scientific Foundation of Northeast Forestry University Grant No. JQ2017-02 (to BN) Natural Science Foundation of Heilongjiang Province Grant No. LH2020C041 (to DW) The Innovation Project of State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University) Grant No. 2019A01 (to BN) National Natural Science Foundation of China Grant No. 61972257 (to XZ).

语种：英文

外文关键词：yellowhorn; phyllosphere bacterial community; climate factors; community composition; microbial networks; keystone taxa

摘要：IntroductionAs an ephemeral and oligotrophic environment, the phyllosphere harbors many highly diverse microorganisms. Importantly, it is known that their colonization of plant leaf surfaces is considerably influenced by a few abiotic factors related to climatic conditions. Yet how the dynamics of phyllosphere bacterial community assembly are shaped by detailed climatological elements, such as various bioclimatic variables, remains poorly understood.MethodsUsing high-throughput 16S rRNA gene amplicon sequencing technology, we analyzed the bacterial communities inhabiting the leaf surfaces of an oilseed tree, yellowhorn (Xanthoceras sorbifolium), grown at four sites (Yinchuan, Otogqianqi, Tongliao, and Zhangwu) whose climatic status differs in northern China.Results and DiscussionWe found that the yellowhorn phyllosphere's bacterial community was generally dominated by four phyla: Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. Nevertheless, bacterial community composition differed significantly among the four sampled site regions, indicating the possible impact of climatological factors upon the phyllosphere microbiome. Interestingly, we also noted that the alpha-diversities of phyllosphere microbiota showed strong positive or negative correlation with 13 bioclimatic factors (including 7 precipitation factors and 6 temperature factors). Furthermore, the relative abundances of 55 amplicon sequence variants (ASVs), including three ASVs representing two keystone taxa (the genera Curtobacterium and Streptomyces), exhibited significant yet contrary responses to the precipitation and temperature climatic variables. That pattern was consistent with all ASVs' trends of possessing opposite correlations to those two parameter classes. In addition, the total number of links and nodes, which conveys community network complexity, increased with rising values of most temperature variables. Besides that, remarkably positive relevance was found between average clustering coefficient and most precipitation variables. Altogether, these results suggest the yellowhorn phyllosphere bacterial community is capable of responding to variation in rainfall and temperature regimes in distinctive ways.