文献类型：期刊文献

英文题名：A cDNA microarray analysis of the molecular control of poplar wood properties

作者：Huang, Qinjun[1,2] Ding, Changjun[1,2] Zhang, Weixi[1,2] Zhang, Bingyu[1,2] Chu, Yanguang[1,2] Wang, Dahai[1,2] Su, Xiaohua[1,2]

第一作者：Huang, Qinjun;黄秦军

通信作者：Su, XH[1];Su, XH[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]State Forestry Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China

年份：2017

卷号：28

期号：1

起止页码：71-82

外文期刊名：JOURNAL OF FORESTRY RESEARCH

收录：;Scopus(收录号：2-s2.0-84981234373);WOS:【SCI-EXPANDED(收录号:WOS:000393585100008)】；

基金：This work was supported by the State Key Basic Research Program of China (No. 2012CB114506).

语种：英文

外文关键词：Populus deltoides; cDNA microarray; Expression profile; Wood density; Microfibrillar angle

摘要：Molecular biological research into wood development and formation has been the focus in recent years, but the pace of discovery of related genes and their functions in the control of wood properties has been slow. The microarray technique-with its advantages of high throughput capacity, sensitivity, and reliability over other tools developed for investigating genes expression patterns-is capable of rapidly assaying thousands of genes. In this study, a cDNA microarray prepared from two cDNA libraries of developing poplar xylem tissues was used to assay gene expression patterns in immature xylem tissues at different heights from the main stem of Populus deltoides (15 years old), which was confirmed to have distinct wood properties (microfibrillar angle, woody density) by X-ray. Two hundred seventy-four transcripts with differential expression profiles between the chips were screened out, and the individual clones were subjected to 5' sequencing. Using bioinformatic analysis, we identified candidate genes that may influence poplar wood properties, many of which belong to various regulatory and signal transduction gene families, such as zinc finger protein transcription factor, DNA-binding transcription factor, ethylene response factors, and so on. The results suggest that these genes may regulate enzymes involved in wood formation. Further work will be performed to clone these genes and determine how they influence poplar wood properties.