文献类型：期刊文献

英文题名：Dynamic changes of heterogeneous cell wall macromolecules in differentiating conifer xylem using cytochemical localization

作者：Wang, Jie[1,2,3,4] Gao, Jie[2] Guo, Juan[1,4] Ma, Lingyu[1,4] Jiang, Xiaomei[1,4] Yu, Hong[3] Li, Jiatao[3] Hu, Yao[3] Daniel, Geoffrey[2] Yin, Yafang[1,4]

第一作者：Wang, Jie

通信作者：Yin, YF[1];Daniel, G[2]

机构：[1]Chinese Acad Forestry, Res Inst Wood Ind, Dept Wood Anat & Utilizat, Beijing 100091, Peoples R China;[2]Swedish Univ Agr Sci, Dept Forest Biomat & Technol Wood Sci, S-75651 Uppsala, Sweden;[3]Chengdu Prod Qual Supervis Inspect & Res Inst, Chengdu 610100, Peoples R China;[4]Natl Forestry & Grassland Adm, Wood Specimen Resource Ctr WOODPEDIA, Beijing 100091, Peoples R China

年份：2025

卷号：284

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20244917468433);Scopus(收录号：2-s2.0-85210531106);WOS:【SCI-EXPANDED(收录号:WOS:001371758000001)】；

基金：This work was supported by the National Natural Science Foundation of China (grant no. 32071691) , and the National Forestry and Grassland Administration (grant no. 2020132601) . JW was supported by the China Scholarship Council (CSC grant no. 202003270032) . We would like to express our gratitude to all the colleagues at Department of Forest Bio-materials and Technology/Wood Science, Swedish University of Agricultural Sciences, and Department of Wood Anatomy and Utilization, Research Institute of Wood Industry, Chinese Academy of Forestry.

语种：英文

外文关键词：Xylem differentiation; Cell wall; Tracheid; Ray; Immunogold labeling; Glucomannan; Xylan; Histochemistry

摘要：Tracing dynamic changes of heterogeneous cell wall components during xylem differentiation is essential for understanding the intricate architecture of wood cell walls at the individual secondary cell wall layer level. Here we employ histochemical- and immunological approaches to visualize the deposition of cellular polymers during xylem differentiation in Pinus bungeana. In axial tracheids, deposition of crystalline cellulose and glucomannan preceded xylan and lignin. Lignification was initiated in primary cell wall corners during development of the S1 layer and intensified with cell wall thickening. Immunofluorescence labeling showed an earlier deposition of glucomannan than xylan with strong presence in S1 layer corner regions at early stages of differentiation. Quantification of immunogold-labeled xylan and glucomannan showed distinct increasing trends during thickening of tracheid wall layers with xylan labeling of the S1 and S2 layers at the S3 stage greater than the S2 stage. Differential cell wall polymer deposition was evident in mature tracheid areas with glucomannan absent in warty layers. Pectins were highly concentrated in unlignified primary cell walls but decreased with axial tracheid wall differentiation. The sequence of polymer deposition in ray cells was similar but lagged behind axial tracheids with ray parenchyma remaining unlignified with thinner cell walls than ray tracheids.