文献类型：期刊文献

中文题名：毛白杨纤维素合成酶基因(PtoCesA1)克隆、序列分析及其植物表达载体的构建

英文题名：Study on Cellulose Synthase Gene of Chinese White Poplar:Cloning,Sequencing and Construction of Higher Plant Expression Vector

作者：李春秀[1] 齐力旺[1] 王建华[1] 张守攻[1]

第一作者：李春秀

机构：[1]中国林业科学研究院林业研究所细胞生物学实验室

年份：2006

卷号：26

期号：2

起止页码：49-52

中文期刊名：中国生物工程杂志

外文期刊名：China Biotechnology

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家"863"计划资助项目(2003AA244060);(2003AA244020)

语种：中文

中文关键词：毛白杨;纤维素合成酶基因;植物表达载体

外文关键词：Chinese white poplar Cellulose synthase gene Higher plant expresstion vector

分类号：Q785

摘要：以毛白杨形成层为材料克隆了毛白杨纤维素合成酶基因（PtoCesA1），序列分析表明该基因序列为3215bp，与欧洲颤杨的PtCesA1基因同源性为97％具有开放的阅读框，编码区在52～2985碱基之间，编码区为2925bp。通过同义突变引入BamHI酶切位点，将全长基因克隆到植物表达栽体pBll21中，经酶切和PCR鉴定确认载体构建正确，为下一步ptoCesA1转基因功能研究打下了基础。
The DNA fragment was cloned from the eDNA that was synthesized using the RNA from Chinese white poplar and verified by sequencing This eDNA clone , designated PtoCesA1, was 3215 bp long with an opening reading frame of 2934bp extending from nucleotides 52 - 2985. Comprision of the nucleotide sequence of PtoCesA1 with PtrCesA1 showed 97% identity. For construction of the PtoCesA1 binary expression vector, the BamHI site was made by synonymy mutation, the full length PtoCesA1 eDNA was subcloned into pBI121 between the BamHI site and Xbal site. The PtoCesA1 binary vector,containing PtoCesA1, was verified by digestion and PCR.